吡格列酮对前脂肪细胞分化及GILZ蛋白表达的影响  

Effect of pioglitazone on pre-adipocytes differentiation and GILZ expression

作　　者：王毅飞[1] 伊娜[2] 吴琳英[1] 黄晓淳[1] 梁思虹 

机构地区：[1]广州医科大学附属第一医院内分泌科,广东广州510120 [2]广州市中医院内分泌科,广东广州510130

出　　处：《中国病理生理杂志》2016年第6期1122-1126,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划(No.2013B021800195);广东省医学科学技术研究基金资助项目(No.A2014291)

摘　　要：目的:探讨吡格列酮在3T3-L1前脂肪细胞分化及糖皮质激素诱导亮氨酸拉链(GILZ)蛋白表达调节方面的作用。方法:形态观察3T3-L1细胞分化过程,不同浓度吡格列酮(1×10^(-4)mmol/L^1×10^(-2)mmol/L)处理细胞48 h,然后于分化的第2、4、6天用油红O染色测定细胞甘油三酯相对含量,实时荧光定量PCR法检测过氧化物增殖活化受体(PPAR)γ2和脂蛋白酶(LPL)的mRNA表达。不同浓度药物处理细胞48 h后,Western blot检测GILZ蛋白表达。结果:油红O法显示甘油三酯相对含量随药物处理浓度的增加而增加,与对照组比,1×10^(-3)mmol/L和1×10^(-2)mmol/L组甘油三酯含量显著性增加(P<0.05)。实时荧光定量PCR检测显示PPARγ2、LPL的mRNA表达也是随着药物处理浓度的增加而增加。与对照组比,吡格列酮高于1×10^(-3)mmol/L浓度时,PPARγ2、LPL的mRNA表达显著性增加(P<0.01)。Western blot显示GILZ蛋白表达随着药物处理浓度的增加而降低。结论:吡格列酮可下调GILZ表达,上调PPARγ2及下游LPL的表达。AIM： To investigate the roles of pioglitazone on differentiation and expression of GILZ in 3T3-L1 pre-adipocytes. METHODS： The morphological changes during 3T3-L1 cell differentiation were observed. The cells were treated with pioglitazone at concentrations of 1 × 10- 4~ 1 × 10- 2mmol / L for 48 h,then the relative content of triglyceride were analyzed by oil red O staining at 2nd,4th and 6th day during adipogenesis. The mRNA expression of peroxisome proliferator-activated receptor γ2（ PPARγ2） and lipoprotein lipase（ LPL） was measured by real-time PCR. GILZ protein expression was determined by Western blot after the cells were treated with pioglitazone at concentrations of 1 × 10- 4~ 1 ×10- 2mmol / L for 48 h. RESULTS： Oil-red O staining showed that the relative contents of triglyceride in adipocytes were increased with the increase in the pioglitazone concentration. Compared with the control,the relative contents of triglyceride in group 1 × 10^-3mmol / L and group 1 × 10^-2mmol / L were significantly increased（ P 0. 05）. The mRNA expression of PPARγ2 and LPL was also increased with the increase in the pioglitazone concentration. When pioglitazone concentration was more than 1 × 10^-3mmol / L,compared with the control,the mRNA expression of PPARγ2 and LPL significantly increased（ P 0. 01）. The protein expression of GILZ was decreased with the increase in the pioglitazone concentration.CONCLUSION： Pioglitazone down-regulates GILZ expression,and up-regulate PPARγ2 expression and the downstream functional factor such as LPL.

关 键 词：吡格列酮 糖皮质激素诱导亮氨酸拉链 脂肪细胞分化 过氧化物增殖活化受体γ2 3T3-L1细胞 

分 类 号：R363[医药卫生—病理学]