牙本质基质对牙囊细胞增殖分化影响的体外研究  

Dentin matrix enhances osteo/cementoblastic differentiation of human dental follicle cells

作　　者：杨禾丰[1,2] 李慧 孙晶晶 郭维华 田卫东 李松[1] 

机构地区：[1]昆明医科大学附属口腔医院,云南昆明650031 [2]口腔再生医学国家地方联合工程实验室,四川成都610041

出　　处：《牙体牙髓牙周病学杂志》2016年第6期338-342,共5页Chinese Journal of Conservative Dentistry

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项资金(2013FZ279);昆明医科大学2015年博士研究生创新基金(2015D03)

摘　　要：目的：研究牙本质基质（DM）对人牙囊细胞（h PFCs）增殖、分化的影响。方法：酶消化组织块法分离培养hDFCs,经鉴定后取第4代细胞并将其随机分为2组,分别用DM浸提液（实验组）和α-DMEM培养液（对照组）进行培养。然后分别用CCK-8法检测各组细胞在培养后1~7 d各时间点的增殖情况;用Western-blot检测培养7 d后各组细胞中ALP、Runx2、OPN、CP-23各成骨/成牙骨质相关基因的蛋白表达水平。结果：DM浸提液对h DFCs的增殖无明显促进作用（P〉0.05）,但能明显上调其ALP、Runx2、OPN、CP-23蛋白表达水平（P〈0.05）。结论：牙本质基质浸提液可诱导hDFCs向成骨/成牙骨质向分化。AIM： To evaluate the effects of dentin matrix（ DM） on the proliferation and osteo/cementoblastic differentiation of human dental follicle cells（ h DFCs） in vitro. METHODS： Liquid extraction of DM was prepared with DM particles. h DFCs were isolated and cultivated,subsequently cultured in DM extraction. The proliferation of h DFCs was evaluated by CCK-8,and the osteo / cementoblastic differentiation of h DFCs was investigated by detection of ALP,Runx2,OPN,CP-23 using Western blot after 7day co-culture. RESULTS： DM did not show the effect on the proliferation of h DFCs（ P〉0. 05）,but it increased the expression of ALP,Runx2,OPN and CP-23 after 7d co-culture（ P〈0.05）. CONCLUSION： Dentin matrix can induce the osteo/cementoblastic differentiation of h DFCs.

关 键 词：人牙囊细胞(hPDFCs) 增殖 分化 牙本质 

分 类 号：R780.2[医药卫生—口腔医学]