麻疯树G蛋白γ亚基JcAGG3基因的克隆及表达分析  

Cloning and Expression Analysis of JcAGG3,G-protein Gamma Subunitsthree Gene from Jatrophacurcas L.

作　　者：张敏[1] 田银帅[1] 胡晓乐[1] 徐莺[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《中国生物工程杂志》2016年第5期46-52,共7页China Biotechnology

基　　金：国家"十二五"重大科技支撑计划资助项目(2011BAD22B08)

摘　　要：从麻疯树c DNA中克隆到一个与拟南芥AGG3同源的基因,命名为Jc AGG3。该基因开放阅读框为834bp,编码277个氨基酸,软件预测等电点为8.61,分子质量为30.514k Da。亚细胞定位预测显示其定位于细胞质膜上。在该基因的顺势作用元件上发现了与胚乳发育、激素调节、光响应和逆境胁迫相关的启动元件。荧光定量PCR(q PCR)检测发现,Jc AGG3在根、茎、叶和种子中均有表达,在发育中的种子中表达量最高,幼叶中的表达量显著高于老叶,在茎中只检测到极微量的表达;对麻疯树的幼苗进行黑暗处理后Jc AGG3基因表达显著下调,脱落酸(ABA)和干旱胁迫处理下Jc AGG3表达量显著增加。An Arabidopsis thaliana AGG3 homologous gene was cloned from Jatrophacurcas L.,which was named Jc AGG3. Jc AGG3 had an open reading frame of 834 bp,encoding a protein of 277 amino acids. Its theoretical isoelectricand molecular weight point were 30. 514 k Da and 8. 61 respectively. The protein was predictedlylocalised in plasma membrane. Through Biologysoftwareprediction analysis,a lot of promoter-binding elements related to the development of endosperm,hormonal regulation,light response and abiotic stresses were found in the cis-acting elements of the gene. The expression of Jc AGG3 in different tissues and different development stages were examined using real-time polymerase chain reaction（ RT-PCR）. The results showed that Jc AGG3 was expressed in root,stem,leaf and seed,with the highest expression in developing seed but least in stem. Young lealves had a significantly higher expression level than older leaves. After 6h dark treatment,the level of Jc AGG3 expression was significantly reduced. However, under abscisicacid（ ABA） and drought treatment,the level of Jc AGG3 expression were increased.

关 键 词：麻疯树 AGG3 种子大小 基因克隆 表达分析 

分 类 号：Q789[生物学—分子生物学]