基于CO I及Cyt b基因的6种蜚蠊分子系统关系  

Phylogenetic Relationship of 6 Blattaria Species Based on Mitochondrial CO I and Cyt b Genes Sequences

作　　者：陈壮志 何苗 张成桂 杨红 闫爽 赵海荣 李成功 巫秀美 

机构地区：[1]大理大学昆虫生物医药研究院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000 [3]大理大学,中国西南药用昆虫及蛛形类资源开发利用协同创新中心,云南大理671000

出　　处：《广州化工》2016年第10期48-51,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金(81360679);药用特种昆虫开发国家地方联合工程研究中心创新能力建设项目(项目编号:云发改高技[2015]395);云南省战略性新兴产业项目发展专项资金([2013]1527);云南省高新技术产业发展重点项目([2012]1956);云南省2011协同创新中心项目([2012]25);云南省昆虫生物医药研发重点实验室专项经费(项目编号:2015DG030)

摘　　要：对美洲大蠊等6种蜚蠊的线粒体CO I和Cyt b基因序列进行了分析,6种蜚蠊的CO I基因片段长度为659 bp,共存在221个核苷酸位点变异,变异率为33.54%;6种蜚蠊的Cyt b基因片段长度均为433 bp,共存在154个核苷酸位点变异,变异率为35.57%。本文以蜚蠊目近缘昆虫Neocapritermes taracua为外群,对CO I和Cyt b两个基因合并序列采用Neighbor-Joining法和Minimum Evolution法构建分子系统树。结果显示:两种方法均能区分美洲大蠊和另外5种蜚蠊,美洲大蠊和黑胸大蠊亲缘关系比较近。Mitochondrial CO I and Cyt b genes sequences of 6 Blattaria species were analyzed. The results showed that the CO I gene segments length was 659 bp for studied insects. There were 224 nucleotide variation sites with the variation rate of 33. 54%. The length of Cyt b gene segments were 433 bp,containing 154 nucleotide variation sites with a variation rate of 35. 57%. Neocapritermes taracua,as the outgroup,used Neighbor- Joining method and Minimum Evolution method to study the union sequences of CO I and Cyt b and to construct Blattaria＇ s molecular phylogenetic trees. It was suggested that the 2 methods analyses could be used in species identification of Periplaneta Americana and its closely related species.Accordant with the result derived from traditional classification system,our study revealed that the genetic relationship of Periplaneta americana and Periplaneta fuliginosa was nearer than with others.

关 键 词：美洲大蠊 线粒体 CO I CYT b 分子系统树 

分 类 号：TQ460[化学工程—制药化工]