小鼠肝切除术后肝种植瘤模型的建立  

Construction of mouse model of liver implanted tumor after hepatectomy

作　　者：张俊斌[1] 汪根树[1] 周静[2] 冯智英[2] 刘慧玲[3] 谌小龙[1] 李慧[1] 王励[1] 李势辉[1] 杨建旭[1] 

机构地区：[1]中山大学附属第三医院器官移植中心,广州510630 [2]中山大学附属第三医院病理科,广州510630 [3]中山大学附属第三医院消化内科,广州510630

出　　处：《中华肝脏外科手术学电子杂志》2016年第3期190-194,共5页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：广东省科技计划项目(2012B060300012);广州市科技计划项目(2014J4100128)

摘　　要：目的探讨肝癌组织块种植法小鼠肝切除术后肝种植瘤模型的建立。方法小鼠H22肝癌细胞接种至2只BALB/c(nu/nu)裸鼠肩胛部,2周后成瘤。按照随机数字表法将18只裸鼠分为无肝切除组(A组),30%肝切除组(B组)和70%肝切除组(C组),每组6只。建立裸鼠肝切除模型,A组裸鼠游离肝左外叶及中叶,B组结扎切除肝左外叶组织,C组结扎切除肝左外叶及中叶组织。取肩胛部肿瘤组织接种至3组裸鼠肝脏。比较3组成瘤率和种植瘤体积。成瘤率比较采用Fisher确切概率法。3组种植瘤体积比较采用Kruskal-Wallis秩和检验及Bonferroni法。结果 2周后A、B、C组的成瘤率分别为6/6、6/6、5/6,差异无统计学意义(P=1.000);A、B、C组的种植瘤体积中位数分别为19(6~33)、22(8~44)和150(58~186)mm^3,C组明显大于A组和B组(Z=9.03,7.12;P<0.0167)。组织病理学检查证实3组种植瘤均为肝细胞癌,伴有不同程度的坏死和炎症细胞浸润。结论肝癌组织块种植法可以建立理想的小鼠肝大部分切除术后肝种植瘤模型,具有实验周期短,成瘤率高以及稳定性好等优点。Objective To explore the construction of mouse model of liver implanted tumor after hepatectomy by implantation of liver tumor tissue.Methods H22 hepatocellular carcinoma（HCC） cells of mice were implanted to the scapula of 2 BALB/c（nu/nu） nude mice and tumors developed in 2 weeks.Eighteen nude mice were divided into three groups according to random number table method：non-hepatectomy group（group A）,30% hepatectomy group（group B） and 70% hepatectomy group（group C） with 6 mice in each group.The hepatectomy model of nude mice was established.The left lateral lobe and middle lobe were dissected for the nude mice in group A.The tissues of left lateral lobe were ligated and resected in group B.And the tissues of left lateralobe and middle lobe were ligated and resected in group C.The tumor tissues incised from the scapula were implanted to the livers of nude mice in three groups.The tumor formation rate and the volume of implanted tumors of three groups were compared.The tumor formation rate was compared using Fisher＇s exact probability test,and the volume of implanted tumors was compared using Kruskal-Wallis test and Bonferroni method.Results The tumor formation rate of group A,B and C after 2 weeks was respectively 6/6,6/6 and 5/6,and no significant difference was observed（P=1.000）.The median volume of implanted tumors in group A,B and C was respectively 19（6-33）,22（8-44） and 150（58-186） mm^3.The median volume of implanted tumors in group C was significantly larger than those in group A and B（Z=9.03,7.12;P〈0.0167）.The implanted tumors in three groups were all confirmed as HCC and with different degrees of necrosis and inflammatory cell infiltration by histopathological examination.Conclusions Implantation of liver tumor tissue can be used to construct an ideal mouse model of liver implanted tumor after major hepatectomy with the advantages of short experimental period,high tumor formation rate and good stability.

关 键 词：肝切除术 癌 肝细胞 肿瘤复发 局部 肿瘤种植 小鼠 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]