鞣花酸对胰腺癌细胞株PANC-1增殖和凋亡的影响及其机制  

Study on effect and mechanism of ellagic acid on the proliferation and apoptosis of pancreatic cancer cell line PANC-1

作　　者：程浩[1] 卢成林[1] 潘一明[1] 唐日波 包善华[1] 仇毓东[1] 谢敏[1] 

机构地区：[1]南京大学医学院附属鼓楼医院普通外科,210008

出　　处：《中华实验外科杂志》2016年第5期1244-1246,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察鞣花酸（EA）对胰腺癌细胞株PANC-1增殖、凋亡的影响,探讨鞣花酸诱导PANC-1凋亡的机制.方法 用不同浓度的鞣花酸（0、2、4、6、8μg/ml）干预PANC-1细胞不同时间（24、48、72 h）后,采用倒置显微镜观察细胞形态变化,噻唑蓝（MTT）比色法检测细胞增殖.取0、2、4、6 μg/ml的鞣花酸干预PANC-1细胞48 h后,细胞染色观察细胞凋亡的形态改变,流式细胞仪检测细胞凋亡率和周期,Western blot法检测环氧合酶-2（COX-2）和核因子-κB （NF-κB）蛋白的表达.结果 鞣花酸对PANC-1细胞有明显的生长抑制作用,呈现明显的浓度依赖性和时间依赖性,不同浓度组、不同时间组间增殖抑制率差异有统计学意义（P＜0.05）,且加药组贴壁细胞出现皱缩、变圆、脱落.不同浓度的鞣花酸干预PANC-1细胞48 h后,细胞形态发生典型凋亡特征性改变,细胞凋亡率分别为（11.18 ±0.99）％、（20.70 ±2.32）％、（39.68 ±0.69）％,与对照组凋亡率[（0.62±0.23）％]比较,不同浓度差异有统计学意义（P＜0.05）;G0/G1期细胞百分比分别为（48.98±2.36）％、（60.80±1.40）％、（72.82 ±0.59）％,与对照组[（42.78±O.74）％]比较,不同浓度差异有统计学意义（P＜0.05）;Western blot检测提示COX-2和NF-κB的蛋白表达明显降低,且呈浓度依赖性.结论 鞣花酸可抑制胰腺癌细胞PANC-1的增殖、诱导其凋亡,其机制可能与阻滞细胞周期,下调COX-2和NF-κB的蛋白表达有关.Objective To investigate the effect of ellagic acid on the proliferation and apoptosis of pancreatic cell line PANC-1 in vitro and explore possible mechanism of ellagic acid＆#39; s effect on PANC-1 cells.Methods PANC-1 cells were treated with ellagic acid at concentrations of 0,2,4,6,8 μg/ml for 24,48 and 72 h respectively.Inverted Microscope showed cells＆#39; morphological changes.The cell proliferation was determined by methyl thiazolyl tetrazolium （MTT） assay.When the cells were treated with ellagic acid at concentrations of 0,2,4,6 μg/ml for 48 h,the morphological changes of apoptotic cells were observed by cell staining,apoptotic rate and cell cycle were detected by flow cytometry,the protein expression of cyclo-oxgenase 2 （COX-2） and nuclear factor-κB （NF-κB） were measured by Western blotting.Results Ellagic acid could significantly inhibited the growth of PANC-1 cells in a concentration-dependent and time-dependent manner.The difference among these groups were statistically significant（P 〈0.05）.After treatment with ellagic acid,apoptosis of PANC-1 cells was seen.Apoptosis rates in the different concentration groups were respectively （11.18 ± 0.99） %,（20.70 ± 2.32） %,（39.68 ± 0.69） %,the apoptosis rate of control group [（0.62 ± 0.23） %] compared with statistically significant difference among the different concentration groups （P 〈 0.05）.The percentages of G0/G1 phase cells were respectively（48.98±2.36）%,（60.80 ± 1.40）%,（72.82 ±0.59）%,the percentage of control group [（42.78 ±0.74）%] compared with statistically significant difference among the different concentration groups（P 〈0.05）.The protein expression of COX-2 and NF-κB measured by Western blotting were significantly down-regulated dose-dependently.Conclusion Ellagic acid can inhibit the proliferation of pancreatic cancer cell line PANC-1 and induce apoptosis.The mechanism may depend on arresting cell cycle and decreasing protein expression of COX-2 and NF-κB.

关 键 词：鞣花酸 胰腺肿瘤 增殖 凋亡 环氧合酶-2 核因子-ΚB 

分 类 号：R735.9[医药卫生—肿瘤]