瞬时受体电位M8离子通道在前列腺癌DU145细胞表达及其激动剂对细胞增殖迁移的影响  

Expression of transient receptor potential melastatin 8 in prostate cancer DU145 cells and effect of transient receptor potential melastatin 8 ion channel agonist on cell proliferation and migration

作　　者：李靖[1] 任君凯[1] 田沛[1] 冯超杰[1] 何朝宏[1] 

机构地区：[1]郑州大学附属肿瘤医院(河南省肿瘤医院)泌尿外科,450008

出　　处：《中华实验外科杂志》2016年第5期1278-1280,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察瞬时受体电位M8离子通道（TRPM8）在前列腺癌DU145细胞表达及TRPM8激动剂对细胞增殖迁移的影响.方法 使用反转录-聚合酶链反应（RT-PCR）、免疫组织化学和Western blot方法检测TRPM8和瞬时受体电位A1离子通道（TRPA1）在前列腺癌旁组织、癌组织和DU145细胞中的表达,使用噻唑蓝（MTT）和划痕试验检测TRPM8受体激动剂薄荷醇对DU145细胞细胞增殖与迁移的影响,使用流式细胞术检测TRPM8对DU145细胞周期与凋亡的影响.结果 TRPM8在前列腺癌DU145细胞中呈明显高表达,TRPA1在DU145细胞中不表达;25、50、75、100 μmol/L浓度薄荷醇处理后,DU145相对细胞数量明显低于空白组[（90.01±8.52）％比（83.24±7.21）％比（71.23±6.45）％比（53.12±5.22）％比（100.00±3.26）％]（P＜0.05）;100 μmol/L薄荷醇处理24、48和72 h后,DU145细胞中G0/G1期细胞明显高于未处理时[（60.98±7.21）％比（76.49±8.12）％比（72.03±7.65）％比（50.26±6.41）％] （P ＜0.05）;100 μmol/L TRPM8激动剂处理24h和48 h后,DU145的细胞迁移率明显低于空白对照组[（58.59±5.24）％比（48.09±4.23）％]比（100.00±3.54）％]（P ＜0.05）.结论 TRPM8在DU145细胞中高表达,TRPM8激动剂能诱导细胞周期阻滞于G0/G1期,进而抑制肿瘤细胞的增殖及迁移.Objective To observe the expression of transient receptor potential melastatin 8 （TRPM8） in prostate cancer DU145 cells and the effect of TRPM8 agonist on cell proliferation and migration.Methods The expression levels of TRPM8 and transient receptor potential ankyrin 1 （TRPA1） in prostate cancer tissue,adjacent prostate tissues and DU145 cells were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）,immunohistochemistry and Western blotting.The effect of TRPM8 agonists menthol on proliferation and motility of DU145 cells were examined by MTT assay and scratch motility assay,and the effect of TRPM8 on cell cycle and apoptosis of DU145 cells was tested by flow cytometry detection.Results TRPM8 was significantly up-regulated,and no TRPA1 expression was detectable in prostate cancer DU145 cell.After treatment with 25,50,75 and 100 μ mol/L menthol,DU145 relative cell number was significantly less than in the blank group [（90.01 ± 8.52） % vs.（83.24 ± 7.21） % vs.（71.23 ±6.45）% vs.（53.12±5.22）% vs.（100.00 ±3.26）%] （P〈0.05）.After treatment with 100 μmol/L menthol for 24,48 and 72 h,the proportion of G0/G1 phase DU145 cells was significantly higher than the untreated [（60.98 ± 7.21） % vs.（76.49 ± 8.12） % vs.（72.03 ± 7.65） % vs.（50.26 ± 6.41） %] （P 〈 0.05）.After treatment with 100 μmoL/L TRPM8 agonist for 24 h and 48 h,motility rate of DU145 cells was significantly lower than in the blank control group [（58.59 ±5.24） % vs.（48.09 ± 4.23） % vs.（100.00 ± 3.54） %] （P 〈 0.05）.Conclusion The TRPM8 was over-expressed in prostate cancer DU145 cells.TRPM8 agonist can arrest cell cycle in G0/G1 phase,and inhibit proliferation.and motility of tumor cells.

关 键 词：瞬时受体电位M8离子通道 前列腺癌 增殖 迁移 

分 类 号：R737.25[医药卫生—肿瘤]