人前列腺癌PC-3细胞系再表达雄激素受体对生长因子相关基因族表达的影响  

Re-expression of androgen receptor(AR) in human prostate cancer cell line PC-3 affects expression patterns of the cluster of growth factor-related genes

作　　者：曹靖晨 张冲[1] 王海燕[1] 张永辉[1] 陈苗苗[1] 吕秀芳[1] 鄂群[1,2] 刘欣[3] 江明[1] 

机构地区：[1]南通大学医学院基础医学研究室核受体与肿瘤研究实验室,南通226001 [2]南通大学医学院病理学系 [3]江苏省靖江市人民医院泌尿外科

出　　处：《中国男科学杂志》2016年第3期3-8,共6页Chinese Journal of Andrology

基　　金：国家自然科学基金(NSFC)面上项目(项目批准号:81372772);江苏特聘教授科研基金(苏教师[2012]34号);南通大学研究生科技创新计划项目(项目编号:YKC14054);江苏高校优势学科建设工程资助项目(PAPD)资助

摘　　要：目的研究雄激素受体(AR)阴性人前列腺癌PC-3细胞系再表达人全长AR cDNA,即PC-3-AR+细胞系生长因子相关基因族的表达情况,探讨雄激素依赖型和非依赖型前列腺癌细胞AR与生长因子信号途径的相关性。方法采用PCR芯片技术(PCR-array)进行研究,利用德国Qiagen公司的RT^2 Profiler^(TM)PCR Array Human Growth Factors板对PC-3、PC-3-AR+两株细胞系的生长因子相关基因族的表达量进行相对定量分析,之后通过数据分析,比较基因表达的差异。结果两株人前列腺癌细胞系PC-3和PC-3-AR+中生长因子相关基因族的表达有明显差异性,其中FGF13、IGF2、CSF2、CXCL1等基因表达差异倍数超过5倍以上,具有显著性差异(P<0.01)。结论人前列腺癌细胞系PC-3再表达雄激素受体AR影响其生长因子相关基因族的表达,人前列腺癌AR与生长因子表达信号通路具有相关性。Objective To investigate the different expressions of growth factor-related genes between human prostate cancer cells PC-3 and PC-3-AR＋ with androgen receptor （AR） re-expressed and discuss the relationship between AR and the signal pathways of growth factors. Methods The different expressions of growth factor-related genes between in human prostate cancer cell lines PC-3 and in PC-3-AR＋ were measured by RT2 ProfilerTM PCR Array Human Growth Factors（Qiagen, Germany）. Results Different expressions of some growth factor-related genes were identified between in human prostate cancer cell lines PC-3 and in PC-3-AR＋, such as FGF13, ERAP1, IGF2, CSF2 and CXCL1 genes （P〈0.01）. Conclusion The re-expression of full-length AR cDNA in human prostate cancer cell line PC-3 may influence the expression levels of growth factor-related genes, indicating that AR may be related to the signal pathways of growth factors in prostate cancer ceils.

关 键 词：前列腺肿瘤 受体 雄激素 生长因子 

分 类 号：R737-25[医药卫生—肿瘤]