中药砂仁、虎杖及桂枝萃取液对乳腺癌细胞MCF-7增殖的抑制作用  

Inhibitory effects of fructus amomi,polygonum cuspidatum sieb and cinnamomi ramulus on the proliferation of MCF-7 breast cancer cells

作　　者：赵光[1] 李珺[1] 杨捷[1] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100069

出　　处：《中国医学装备》2016年第6期94-98,共5页China Medical Equipment

基　　金：国家自然科学基金(NO30500648)"以FAS为靶从元宝枫发展无耐药性的抗肿瘤药物";北京市自然科学基金资助(NO7103168)"以介孔材料为载体从天然产物发展抗肿瘤靶向给药系统";北京市教育委员会科技发展计划面上项目(KM201010025011)"从虎杖等天然产物中发展肿瘤化学预防药物";首都中医药研究专项(ZM02)"医院制剂抗瘤丸治疗神经胶质瘤的作用机理研究"

摘　　要：目的:筛选具有体外抑制脂肪酸合酶活性的天然产物,研究中药砂仁、虎杖及桂枝萃取液对MCF-7乳腺癌细胞的抑制作用。方法:将细胞株按照不同中药萃取液处理方式分为实验组与对照组,实验组中分为虎杖组、白藜芦醇组、虎杖苷组、砂仁组以及桂枝组;对照组以无血清RMPI1640培养液代替药物。采用CCK-8法观测不同浓度的砂仁、虎杖及桂枝以及其主要成分白藜芦醇和肉桂酸,作用24 h后对MCF-7乳腺癌细胞增殖的影响。结果:砂仁、虎杖及桂枝中60%乙醇提取物IC50分别为24.86μg/ml、153.67μg/ml及178μg/ml;白藜芦醇的抑癌活性强于虎杖,IC50为61.75μg/ml;桂枝的主要成分肉桂酸在低浓度时抑癌活性强。结论:砂仁、虎杖及桂枝60%乙醇提取物均能显著抑制MCF-7乳腺癌细胞生长,且呈现出浓度依赖性,其中砂仁活性最强,效果最显著。Objective：To screen fatty acid synthase （FAS） inhibitors from natural products and study their inhibitory effects on the proliferation of MCF-7 breast cancer cells.Methods： CCK-8 method was used to detect the inhibitory effects of Fructus Amomi, Polygonum cuspidatum Sieb, Cinnamomi Ramulus and their main compounds, such as polydatin, resveratrol and cinnamic acid on the proliferation of MCF-7 breast cancer cells for 24 h.Results： The results showed that the IC50 values of the 60% ethanol extracts of Fructus Amomi, Polygonum cuspidatum Sieb, and Cinnamomi Ramulus were 24.86μg/ml, 153.67 μg/ml and 178 μg/ml respectively. The IC50 value of Resveratrol was 61.75 μg/ml. The inhibitory effect of Resveratrol was better than that of Polygonum cuspidatum Sieb. Cinnamic acid, the main component of Cinnamomi Ramulus had better inhibitory activity at lower concentration.Conclusion： The 60% ethanol extracts of Fructus Amomi, Polygonum cuspidatum Sieb, and Cinnamomi Ramulus all showed inhibitory effects on the proliferation of MCF-7 breast cancer cells in a dose-dependent manner. Among them, Fructus Amomi had the best inhibitory activity.

关 键 词：脂肪酸合酶抑制剂 人乳腺癌细胞MCF-7 天然产物 

分 类 号：R737.9[医药卫生—肿瘤]