Characterization of decellularized scaffold derived from porcine meniscus for tissue engineering applications  

作　　者：Shuang GAO Zhiguo YUAN Tingfei XI Xiaojuan WEI Quanyi GUO 

机构地区：[1]Center for Biomaterial and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China [2]Key Laboratory of PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China [3]Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China

出　　处：《Frontiers of Materials Science》2016年第2期101-112,共12页材料学前沿（英文版）

摘　　要：Menisci are fundamental fibrocartilaginous organs in knee joints. The injury in meniscus can impair normal knee function and predisposes patients to osteoarthritis. This study prepared decellularized meniscus scaffolds using a 1% （w/w） sodium dodecyl sulfate solution and sufficient rinsing steps. Complete cell removal was verified by hematoxylin and eosin staining and DNA content assay. Decellularized menisci had accordant tension properties to intact ones, but with declined compression properties. This occurred because the collagen fiber was not damaged but glycosami- noglycans was significantly lost during the decellularization process, which was confirmed by biochemical assay and histology staining. In vitro cytotoxicity assay demonstrated that decellularized meniscus scaffolds have no toxicity on L929 murine fibroblasts and porcine chondrocytes. Further experiment showed that porcine chondrocytes could adhere and proliferate on the scaffold surface, and some cells even could infiltrate into the scaffold. All results showed the potential of this decellularized meniscus to be the scaffolds in tissue engineering.Menisci are fundamental fibrocartilaginous organs in knee joints. The injury in meniscus can impair normal knee function and predisposes patients to osteoarthritis. This study prepared decellularized meniscus scaffolds using a 1% （w/w） sodium dodecyl sulfate solution and sufficient rinsing steps. Complete cell removal was verified by hematoxylin and eosin staining and DNA content assay. Decellularized menisci had accordant tension properties to intact ones, but with declined compression properties. This occurred because the collagen fiber was not damaged but glycosami- noglycans was significantly lost during the decellularization process, which was confirmed by biochemical assay and histology staining. In vitro cytotoxicity assay demonstrated that decellularized meniscus scaffolds have no toxicity on L929 murine fibroblasts and porcine chondrocytes. Further experiment showed that porcine chondrocytes could adhere and proliferate on the scaffold surface, and some cells even could infiltrate into the scaffold. All results showed the potential of this decellularized meniscus to be the scaffolds in tissue engineering.

关 键 词：meniscus scaffold DECELLULARIZATION BIOMECHANICAL cytotoxicity TISSUEENGINEERING 

分 类 号：Q813.11[生物学—生物工程] TB39[一般工业技术—材料科学与工程]