肝肺综合征患者血清对人肺成纤维细胞肌样分化、增殖与迁移的影响  

Effect of serum of patients with hepatopulmonary syndrome on myogenic differentiation, proliferation and migration of human lung fibroblasts

作　　者：曾子洋[1] 高静[1] 陈林[1] 刘畅[1] 易斌[1] 

机构地区：[1]第三军医大学西南医院麻醉科,重庆市400038

出　　处：《中华麻醉学杂志》2016年第3期354-357,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81270510,81170414）

摘　　要：目的 探讨肝肺综合征患者血清对人肺成纤维细胞肌样分化、增殖与迁移的影响.方法 人肺成纤维细胞接种于培养孔或培养瓶,采用随机数字表法分为2组（n=31）：肝肺综合征患者血清组和健康志愿者血清组,分别用含10％肝肺综合征患者血清的DMEM培养基或10％健康志愿者血清的DMEM培养基孵育.于孵育24、48和72 h（T1-T3）时,采用Western blot法检测人肺成纤维细胞平滑肌-α-肌动蛋白（SM-α-actin）和心肌肌凝蛋白（SM-MHC）的表达,采用3H-TDR法检测人肺成纤维细胞增殖能力,采用Transwell小室法检测人肺成纤维细胞迁移能力.结果 与健康志愿者血清组比较,肝肺综合征患者血清组各时点人肺成纤维细胞SM-α-actin和SM-MHC表达上调,T2,3时增殖和迁移能力增强（P＜0.05）;与T1时比较,肝肺综合征患者血清组T2,3时人肺成纤维细胞SM-α-actin和SM-MHC表达上调,增殖和迁移能力增强（P＜0.05）;与T2时比较,肝肺综合征患者血清组T3时人肺成纤维细胞SM-α-actin和SM-MHC表达上调,增殖和迁移能力增强（P＜0.05）.结论 肝肺综合征患者血清可促进人肺成纤维细胞肌样分化、增殖和迁移.Objective To investigate the effect of the serum of patients with hepatopulmonary syndrome on the myogenic differentiation,proliferation and migration of human lung fibroblasts.Methods The human lung fibroblasts were seeded in plates or flasks and randomly divided into 2 groups （n =31each） using a random number table：serum of patients with hepato-pulmonary syndrome group and serum of healthy volunteer group.The human lung fibroblasts were incubated in the DMEM culture medium containing 10% serum of patients with hepatopulmonary syndrome or in the DMEM culture medium containing 10% serum of healthy volunteers.At 24,48 and 72 h of incubation （T1-T3）,the expression of smooth muscle-α-actin （SM-α-actin） and smooth muscle myosin heavy chain （SM-MHC） in human lung fibroblasts was determined by Western blot,the proliferation of the human lung fibroblasts was determined using 3H-TDR incorporation assay,and the migration of the human lung fibroblasts was determined by Transwell chamber assay.Results Compared with serum of healthy volunteer group,the expression of SM-α-actin and SM-MHC in human lung fibroblasts was significantly up-regulated at each time point,and the proliferation and migration of the cells were significantly enhanced at T2,3 in serum of patients with hepatopulmonary syndrome group （P〈0.05）.Compared with the value at T1,the expression of SM-α-actin and SM-MHC in human lung fibroblasts was significantly up-regulated,and the proliferation and migration of the cells were significantly enhanced at T2,3in serum of patients with hepatopulmonary syndrome group （P〈0.05）.Compared with the value at T2,the expression of SM-α-actin and SM-MHC in human lung fibroblasts was significantly up-regulated,and the proliferation and migration of the cells were significantly enhanced at T3 in serum of patients with hepatopulmonary syndrome group （P〈0.05）.Conclusion The serum of patients with hepatopulmonary syndrome can promote the myogenic differentiation,proliferation and migration of

关 键 词：肝肺综合征 成纤维细胞 肺 细胞增殖 细胞运动 细胞分化 

分 类 号：R575.2[医药卫生—消化系统] R563[医药卫生—内科学]