银杏内酯B对星形胶质细胞氧化损伤的保护作用  

Protective Effect of Ginkgolide B on Astrocytes Oxidative Damage from Oxidative Damage

作　　者：刘媛媛[1] 康凯[1] 杨威[1] 彭相虹 杨松柳[1] 赵鸣雁[1] 

机构地区：[1]哈尔滨医科大学附属第一医院重症医学科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2016年第16期3010-3013,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81171785)

摘　　要：目的:探讨银杏内酯B对过氧化氢(H_2O_2)诱导的星形胶质细胞损伤的保护作用及可能机制。方法:星形胶质细胞传代培养,分为阴性对照组(以正常培养液培养),氧化损伤组(100μmol·L^(-1)的H_2O_2作用12 h),银杏内酯B低剂量组(1×10^(-6) mol·L^(-1)银杏内酯B孵育24 h后,加入H_2O_2作用12 h)和银杏内酯B高剂量组(1×10^(-4) mol·L^(-1)银杏内酯B孵育24 h后,加入H_2O_2作用12 h),MTT比色法检测细胞存活率,流式细胞仪检测细胞活性氧(ROS)水平,分光光度计检测上清液中超氧化物歧化酶(SOD)、如谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量。结果:银杏内酯B能抑制氧化损伤引起的细胞活性的下降,降低星形胶质细胞内ROS的生成,促进SOD、GSH-Px水平的升高及MDA水平的下降。结论:银杏内酯B通过提高细胞内SOD、GSH-Px含量,降低细胞内MDA含量发挥其较强的抗氧化作用,从而为其用于治疗神经系统疾病提供可靠的实验依据。Objective： To investigate the protective effect and possible mechanisms of ginkgolide B on astrocytes induced by oxidative damage. Methods： Astrocytes were subcultured and divided into negative control group （cultured with normal medium）, oxidative injury group （100 μmol·L^-1 H2O2 treated for 12 h）, ginkgolide B low dose group （1×10^-6 mol·L^-1 ginkgolide B treated for 24 b, then 100 μmol·L^-1 H2O2 treated for 12 h）, ginkgolide B high dose group （1×10^-4 mol·L^-1 ginkgolide B treated for 24 h, then 100μmol·L^-1 H2O2 treated for 12 h）, cell viability was assayed by MTT colorimetric, reactive oxygen species （ROS） levels were detected by flow cytometry, content of superoxide dismutase （SOD） glutathione peroxidase （GSH-Px） and malondialdehyde （MDA） in supernatants were detected by spectrophotometer. Results： Ginkgolide B could inhibit oxidative damage induced cell activity decline, decreased the generation of ROS in astrocytes, promote the rise of levels of SOD, GSH-Px and decline of MDA. Conclusion： Ginkgolide B play its strong antioxidant effect by increasing the intracellular content of SOD, GSH-Px levels and decreasing MDA levels, which provide reliable experimental basis for treatment of cataracts.

关 键 词：银杏内酯B H2O2 星形胶质细胞 氧化损伤 

分 类 号：R-33[医药卫生] Q593.2[生物学—生物化学]