3种不同来源侧耳品种亲缘关系差异化分析  

Alliance Variance Analysis of Three Pleurotus Species from Different Sources

作　　者：赵同心[1] 赵洪新[2] 王升厚[3] 

机构地区：[1]沈阳师范大学化学与生命科学学院,辽宁沈阳110034 [2]浙江理工大学生命科学学院,浙江杭州310018 [3]沈阳师范大学实验教学中心,辽宁沈阳110034

出　　处：《微生物学杂志》2016年第2期62-66,共5页Journal of Microbiology

基　　金：辽宁省科学事业公益项目(2014003020)

摘　　要：采取拮抗实验、酯酶同工酶、基因组进行多态性分析(RAPD)、内部转录间隔区序列(ITS)对来源不同的特大凤尾菇(Pleurotus sajor-caju)、夏秀990(Pleurotus geesteranus)和黑平A3三个侧耳品种的亲缘关系进行了对比分析。实验结果表明:供试的3个侧耳品种从形态学水平、蛋白质水平和分子水平上看,其品种间亲缘关系界定具有一致性。表现为特大凤尾菇与夏秀990的亲缘关系较近,与黑平A3的亲缘关系较远,夏秀990与黑平A3亲缘关系较远。本研究从不同的水平,为食用菌菌种属间亲缘关系的鉴定提供了综合分析方法,对区分同种异名、杂交亲本选择以及判定杂交结果具有指导意义。In this study the alliance among three Pleurotus of phoenix tail mushroom,xiaxiu 990 and black oyster mushroom A3 from different sources with different morphology were carried out contrast analysis.The results showed that the tested three Pleurotus species possessed the congruity among species alliance definition in morphology level,protein level and molecular level.The alliance between phoenix tail mushroom and xiaxiu 990 displayed closer,but further from black oyster mushroom A3,and the alliance between xiaxiu 990 and black oyster mushroom A3 was further.This study provided comprehensive methods to analyze the characterization of the alliance of edible fungi of intergenus in different levels,and had directive significance to separate different name of the same species,to select hybrid parents,and to judge the results of hybridization.

关 键 词：侧耳 亲缘关系 酯酶同工酶 随机扩增多态性DNA(RAPD) 转录间隔区序列(ITS) 

分 类 号：Q93-331[生物学—微生物学]