GFI-1在慢性髓细胞白血病患者中的表达及意义  

The expression and significance of GFI-1 in patients of chronic myeloid leukemia(CML)

作　　者：朱星星[1] 刘红[1] 

机构地区：[1]南通大学附属医院血液科,江苏226001

出　　处：《交通医学》2016年第2期103-106,共4页Medical Journal of Communications

基　　金：国家自然科学基金面上项目(21175075);江苏省医学创新团队与领军人才项目(LJ201136);南通市社会事业科技创新与示范-临床医学科技专项(HS2013067)

摘　　要：目的:研究慢性髓细胞白血病(CML)患者中GFI-1的表达情况及其与BCR-ABL定量之间的关系,以探讨GFI-1在CML中的意义。方法:收集82例CML患者的骨髓标本,其中初诊慢性期50例,急变期6例,加速期6例,经伊马替尼治疗后细胞遗传学缓解期20例。以20例正常人的外周血或骨髓标本作为正常对照。应用实时定量PCR、Western blot等方法检测CML患者GFI-1、BCR-ABL的表达情况,分析两者基因表达量之间的关系。结果:(1)CML慢性期、急变期/加速期GFI-1的表达均明显高于正常对照组(P<0.05);慢性期与急变期/加速期患者GFI-1的表达差异无统计学意义(P>0.05);慢性期患者的GFI-1较缓解期表达高,差异有统计学意义(P<0.05)。(2)GFI-1与BCR-ABL定量之间成正相关(r2=0.7647,P<0.01)。结论 :CML患者GFI-1表达水平异常,可能参与CML的发生发展。Objective：Study the expression an d significance of GFI-1 in patients of chronic myeloid leukemia（CML）,and evaluate the relationship of GFI-1m RNA and BCR-ABL m RNA,to explore the significance of GFI-1 in CML.Methods：Collect the bone marrow samples of 82 patients of CML which includes 50 cases of chronic phase,6 cases of CML-accelerated phase（AP）,6 cases of blastic phase（BP）,20 cases of Cytogenetics response by Imatinib. And 20 cases of normal peripheral blood or bone marrow samples as controls.Real-time quantitative PCR,Western blot were used to detect the expression of GFI-1、BCR-ABL in CML patients and normal,to further research the ocurrence and progress of CML.Results：（1）GFI-1 expression in CP,AP/BP in CML was significantly higher than controls（P〈0.05）;expresion in CML CP and AP/BP patients was not statistically significant（P〉 0.05）;CP was higher than remission expression and the difference was statistically significant（P〈0.05）.（2）GFI-1m RNA expression was significantly related to BCR-ABL m RNA（r2=0.7647,P〈0.01）. Conclusion： The GFI-1 expression in CML patients was significantly higher than in normal, which may be involved in the development of CML.

关 键 词：独立生长因子1 慢性髓细胞白血病 BCR-ABL融合基因 

分 类 号：R733.7[医药卫生—肿瘤]