骨髓间充质干细胞联合血管内皮祖细胞修复骨质疏松性牙槽骨缺损  

Bone marrow mesenchymal stem cells combined with endothelial progenitor cells for repair of alveolar bone defect in ovariectomized rats

作　　者：文艺 杨鸿旭 刘倩 梁源 刘竹影 丁寅 

机构地区：[1]解放军军事口腔医学国家重点实验室,解放军第四军医大学口腔医院正畸科,陕西省西安市710032 [2]解放军第四军医大学口腔医院口腔解剖生理学教研室,陕西省西安市710032

出　　处：《中国组织工程研究》2016年第19期2748-2755,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81170982)~~

摘　　要：背景:国内外已有多项研究证实雌激素可以调控血管内皮祖细胞的增殖和迁移能力,同时血管内皮祖细胞也可提高体外培养骨髓间充质干细胞的活性与功能。目的:评价骨髓间充质干细胞联合血管内皮祖细胞复合膜片修复去势大鼠牙槽骨缺损的能力。方法:将制备好的骨髓间充质干细胞/血管内皮祖细胞复合膜片、骨髓间充质干细胞膜片、血管内皮祖细胞膜片植入去势大鼠牙槽骨缺损处,或不植入作为空白对照。植入后2,4,8周分别取材,通过Micro-CT扫描的方法评价牙槽骨缺损的修复效果。结果与结论:相对于单一的正常骨髓间充质干细胞膜片以及单一的正常血管内皮祖细胞膜片,正常骨髓间充质干细胞/血管内皮祖细胞复合膜片具有更强的成骨活性以及骨修复能力。BACKGROUND： Numerous studies have demonstrated that estrogen can regulate the proliferation and migration of endothelial progenitor cells（EPCs）, while EPCs can also promote the function and activity of bone marrow mesenchymal stem cells（BMSCs） in vitro. OBJECTIVE： To evaluate the ability of the BMSCs and EPCs which construct the composite cell sheet in the repair of alveolar bone defect in ovariectomized rats. METHODS： BMSCs/EPCs composite sheet, EPCs sheet and BMSCs sheet were respectively implanted into the defects of the alveolar bone in ovariectomized rats. Rats with no implantation served as control group. Repaired alveolar bone was assessed by gross examination, histological observation and micro-CT scan at 2, 4, 8 weeks after operation. RESULTS AND CONCLUSION： BMSCs/EPCs composite sheet has greater osteogensis activity and bone repair capacity than BMSCs or EPCs sheet alone.

关 键 词：骨质疏松 绝经后 牙槽骨质丢失 骨髓 间质干细胞 组织工程 干细胞 骨髓干细胞 细胞膜片 骨髓间充质干细胞 血管内皮祖细胞 共培养 骨质疏松 牙槽骨缺损 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]