瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能  

Rosuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation improves cardiac function after acute myocardial infarction

作　　者：王艳丽[1] 李金峰[1] 王艳萍[2] 高明[1] 

机构地区：[1]唐山市开滦总医院心内科,河北省唐山市063000 [2]唐山市遵化市人民医院,河北省唐山市064200

出　　处：《中国组织工程研究》2016年第19期2796-2802,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来通过干细胞移植诱导梗死区心肌细胞的再生用以治疗急性心肌梗死后心功能不全,成为较为热议的一种新的治疗手段。目的:观察瑞舒伐他汀联合脐血间充质干细胞移植对大鼠急性心肌梗死后心功能的改善作用。方法:选取45只SD大鼠采用结扎左冠状动脉前降支方法制备心肌梗死模型,随机分为模型组、脐血间充质干细胞移植组和联合组,每组15只。大鼠冠脉结扎后第7天,脐血间充质干细胞移植组及联合组经尾静脉注射脐血间充质干细胞悬液300μL(15.0×108个细胞),模型组注射相同剂量LG-DMEM培养液,联合组给予瑞舒伐他汀1 mg/kg灌胃×28 d(从术后第1天开始灌胃,1次/d),脐血间充质干细胞移植组及模型组同步灌胃相同剂量的生理盐水。造模后5周(细胞移植4周)采用彩色多普勒超声仪检测心功能改善情况,酶联免疫吸附法检测血浆Lp-PLA2水平,苏木精-伊红染色观察心肌组织病理变化;Western blot方法检测心肌梗死区热休克蛋白70表达。结果与结论:1联合组大鼠的左心室射血分数、左心室收缩末压明显高于脐血间充质干细胞移植组(P<0.05),脐血间充质干细胞移植组明显高于模型组(P<0.05);联合组大鼠的左心室舒张末压明显低于脐血间充质干细胞移植组(P<0.05),脐血间充质干细胞移植组明显低于模型组(P<0.05);2联合组Lp-PLA2水平明显低于脐血间充质干细胞移植组(P<0.05),脐血间充质干细胞移植组明显低于模型组(P<0.05);3联合组梗死区的心肌细胞数量较模型组、脐血间充质干细胞移植组明显增加;4心肌梗死区HSP70蛋白表达在联合组明显升高,各组间的表达差异有显著性意义(P<0.05);5结果表明,瑞舒伐他汀联合脐血间充质干细胞移植干预后能确切改善心肌梗死大鼠心功能。BACKGROUND： In recent years, it has been a hot topic that stem cell transplantation is used to improve cardiac insufficiency after acute myocardial infarction by inducing regeneration of cardiomyocytes in the infarction regions. OBJECTIVE： To observe the effect of rosuvastatin combined with umbilical cord blood mesenchymal stem cells transplantation on rat cardiac function after acute myocardial infarction. METHODS： Forty-five Sprague-Dawley rats were enrolled to prepare myocardial infarction models by ligaturing the left anterior descending coronary artery. Then they were equivalently divided into model group, transplantation group and combination group. At 7 days after modeling, rats in the combination group were given injection of 300 μL umbilical cord blood mesenchymal stem cells（15.0×10^8） via the tail vein and by gavage once a day for 28 days with 1 mg/kg rosuvastatin; rats in the transplantation group and model group were injected with 300 μL umbilical cord blood mesenchymal stem cell suspension through the tail veins or the same amount of LG-DMEM medium, respectively, followed by intragastrical administration of the same amount normal saline. At 5 weeks after modeling, indexes of cardiac function, level of plasma Lp-PLA2 and heat shock protein 70 in the infarction regions were detected by color Doppler ultrasound, enzyme-linked immunosorbent assay and western blot assay, respectively. In addition, pathological changes of myocardial tissues were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION： Left ventricular ejection fraction and left ventricular end-systolic pressure were significantly higher in the combination group than in the transplantation group as well as higher in the transplantation group than the model group（P〈0.05）; compared with the transplantation group, left ventricular end-diastolic pressure was significantly decreased in the combination group, but significantly increased in the model group（P〈0.05）; the number of cardiomyocytes in the infarction

关 键 词：羟甲基戊二酰基COA还原酶抑制剂 脐血干细胞移植 心肌梗塞 组织工程 干细胞 移植 瑞舒伐他汀 脐血间充质干细胞 细胞移植 急性心肌梗死 心功能 

分 类 号：R394.2[医药卫生—医学遗传学]