人脐血间充质干细胞移植联合神经节苷脂注射治疗脑性瘫痪  

Human umbilical blood cord mesenchymal stem cell transplantation combined with injection of ganglioside for cerebral palsy

作　　者：杨自金[1] 郭佳丽[1] 卢思广[1] 高长龙[1] 李红梅[1] 冯越[1] 

机构地区：[1]连云港市第一人民医院儿内科,江苏省连云港市222000

出　　处：《中国组织工程研究》2016年第19期2803-2809,共7页Chinese Journal of Tissue Engineering Research

基　　金：江苏省连云港市科技局重点科室建设(SH1117)~~

摘　　要：背景:最近研究证实神经节苷脂在体外能促进脐血间充质干细胞存活及分化。目的:观察侧脑室注射人脐血间充质干细胞及神经节苷脂对脑性瘫痪大鼠神经功能恢复的影响。方法:受孕17 d大鼠腹腔注射脂多糖2 d,放回笼中饲养,孕鼠分娩出脑性瘫痪乳鼠60只,随机分5组:模型组10只,假移植组10只,脐血间充质干细胞移植组18只,神经节苷脂组10只,联合移植组12只。在立体定位仪下,将脐血间充质干细胞或神经节苷脂注入左侧侧脑室,假移植组注入同样体积的PBS。在移植后7,14,21,28 d分别处死2只脐血间充质干细胞移植组大鼠,移植后14 d处死2只联合移植组大鼠,进行脑组织免疫荧光染色。移植后28 d进行神经功能评估。结果与结论:1脐血间充质干细胞能存活、迁移、分化,主要分布在侧脑室、海马、皮质。移植后14 d,联合移植组Brd U、GFAP阳性细胞数明显高于脐血间充质干细胞移植组,差异有显著性意义(P<0.05);2神经功能评估:与模型组比较,脐血间充质干细胞移植组、神经节苷脂组、联合移植组的握持时间延长、足错误次数减少,差异有显著性意义(P<0.05)。联合移植组优于脐血间充质干细胞移植组及神经节苷脂组,差异有显著性意义(P<0.05);3结果表明,脐血间充质干细胞及神经节苷脂均能改善脑性瘫痪大鼠神经功能,神经节苷脂能够提高脐血间充质干细胞在大鼠脑内存活及分化,联合应用效果更好。BACKGROUND： In recent years, some studies have demonstrated that ganglioside can promote survival and differentiation of umbilical blood cord mesenchymal stem cells in vitro. OBJECTIVE： To observe the effect of injection of human umbilical blood cord mesenchymal stem cells and ganglioside into rat lateral ventricles on neurological functional recovery from cerebral palsy. METHODS： Totally 60 cerebral palsy neonatal rats were delivered from pregnant rats which were modes were given intraperitoneal injection of lipopolysaccharide for 2 successive days on day 17 of gestation. Then those neonatal rats were randomly divided into five groups, including model group（n=10）, sham transplantation group（n=10）, stem cell transplantation group（n=18）, ganglioside group（n=10） and combination group（n=12）. Under stereotaxic instrument, umbilical blood cord mesenchymal stem cells or ganglioside were injected into left lateral ventricles of the rat brain, respectively, and the sham transplantation group was given the same volume of phosphate buffered saline. Two rats from the stem cell transplantation group were put to death for immunofluorescence staining at 7, 14, 21 and 28 days after transplantation, respectively, and two rats in the combination group were killed for immunofluorescence staining at 14 days. Besides, all rats were underwent neurologic evaluation at 28 days after transplantation. RESULTS AND CONCLUSION： The umbilical blood cord mesenchymal stem cells could survive, migrate and differentiate, which mainly distributed in the lateral ventricle, hippocampus and cortex. At 14 days after transplantation, positive expressions of Brd U and glial fibrillary acidic protein in the combination group were significantly higher than those in the stem cell transplantation group（P〈0.05）. In addition, compared with the model group, the holding time significantly prolonged and foot error times significantly decreased in the latter three groups（P〈0.05）, as well as in the combination group compare

关 键 词：脑性瘫痪 神经节苷脂类 脐血干细胞移植 组织工程 干细胞 移植 脐血间充质干细胞 神经节苷脂 细胞移植 神经功能 

分 类 号：R394.2[医药卫生—医学遗传学]