葛根芩连汤联合人羊膜间充质干细胞移植治疗糖尿病早期视网膜病变  

Gegen Qin Lian Tang combined with human amniotic mesenchymal stem cell transplantation for early diabetic retinopathy

作　　者：刘亮[1] 王金梁[2] 

机构地区：[1]辽宁中医药大学教师教学发展中心,辽宁省沈阳市110847 [2]辽宁中医药大学附属医院心内科,辽宁省沈阳市110847

出　　处：《中国组织工程研究》2016年第19期2824-2830,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究发现人羊膜间充质干细胞可以向胰岛素分泌细胞分化,还具有免疫调节作用。临床证实葛根芩连汤有明确降低血糖的作用。目的:探讨葛根芩连汤联合人羊膜间充质干细胞移植对糖尿病大鼠早期视网膜病变形态学、相关血清学指标及血管内皮生长因子基因表达的影响。方法:以腹腔注射链脲佐菌素方式制作糖尿病模型,将造模成功的42只Wistar大鼠随机分为模型组、人羊膜间充质干细胞移植组和联合组(人羊膜间充质干细胞移植+葛根芩连汤),移植后4周测定糖尿病大鼠血糖、血清胰岛素水平;移植后4周苏木精-伊红染色镜下观察糖尿病大鼠视网膜的病理形态;免疫组织化学方法检测视网膜组织CD45的表达水平;RT-PCR法检测视网膜血管内皮生长因子m RNA的表达。结果与结论:1与模型组比较,人羊膜间充质干细胞移植组和联合组大鼠血糖降低,血清胰岛素浓度增加,联合组改善更为明显,组间比较差异有显著性意义(P<0.05);2模型组大鼠有明显视网膜水肿、层次紊乱,细胞排列不规则;与模型组比较,人羊膜间充质干细胞移植组大鼠视网膜病变相对轻微,联合组视网膜病变明显好转;3模型组视网膜CD45的表达明显增加,人羊膜间充质干细胞移植组CD45的表达量明显小于模型组(P<0.05),但大于联合组(P<0.05);4联合组和人羊膜间充质干细胞移植组血管内皮生长因子m RNA表达低于模型组,联合组降低幅度更大,差异有显著性意义(P<0.05);5结果表明,葛根芩连汤联合人羊膜间充质干细胞移植可能通过抑制CD45和血管内皮生长因子在视网膜组织表达,从而改善糖尿病视网膜病损严重程度。BACKGROUND： Human amniotic mesenchymal stem cells have the potential to differentiate into insulin-secreting cells and have immunomodulatory effects. Additionally, it has been clinically proved that Gegen Qin Lian Tang can lower blood sugar. OBJECTIVE： To investigate the effects of Gegen Qin Lian Tang combined with human amniotic mesenchymal stem cell transplantation on rat early diabetic retinopathy, by detecting pathological changes, levels of blood indicators and expressions of vascular endothelial growth factor in retinal tissues. METHODS： By intraperitoneal injection of streptozotocin, 42 Wistar rat models with diabetes mellitus were prepared and then randomly divided into model group, human amniotic mesenchymal stem cell transplantation group（cell transplantation group） and combination of human amniotic mesenchymal stem cells and Gegen Qin Lian Tang group（combination group）. At 4 weeks after transplantation, levels of blood sugar and serum insulin in diabetic rats were detected; pathological changes of the retina in diabetic rats were observed by hematoxylin-eosin staining. Besides, expressions of CD45 and vascular endothelial growth factor mRNA were detected using immunohistochemistry staining and RT-PCR technology, respectively. RESULTS AND CONCLUSION： Compared with the model group, the levels of blood glucose and serum insulin in both cell transplantation and combination groups were significantly decreased and increased, respectively, and these changes were even more significant in the combination group（P〈0.05）. In the model group, the retinal edema could be found, accompanied by structural disorder and irregular cell arrangement, while these retinal lesions were relatively milder in the cell transplantation group and significantly improved in the combination group. In addition, the CD45 expression and in the retina was highest in the model group subsequently followed by the cell transplantation group（P〈0.05） and lowest in the combination group（P〈0.05）. Furthermore, comp

关 键 词：葛属 羊膜 间质干细胞移植 糖尿病视网膜病变 组织工程 干细胞 移植 葛根芩连汤 人羊膜间充质干细胞 糖尿病 视网膜病变 血管内皮生长因子 

分 类 号：R394.2[医药卫生—医学遗传学]