骨髓单个核细胞移植治疗脑出血  

Bone marrow mononuclear cell transplantation for cerebral hemorrhage

作　　者：杨郁野[1] 王景丰[1] 张宏义[1] 王跃伍 杨树全 

机构地区：[1]唐山工人医院神经外科,河北省唐山市063000 [2]河北大学附属医院神经外科,河北省保定市071000

出　　处：《中国组织工程研究》2016年第19期2831-2837,共7页Chinese Journal of Tissue Engineering Research

基　　金：河北省医学科学研究重点课题(20130617)~~

摘　　要：背景:研究证实,骨髓单个核细胞移植能够明显改善脑出血大鼠的神经功能。目的:观察骨髓单个核细胞移植对大鼠脑出血后神经功能损伤及血肿脑组织细胞凋亡的影响。方法:将36只SD大鼠随机分为3组,每组12只,正常对照组不进行任何干预;采用立体定向仪向模型组、移植组大鼠尾状核注射Ⅳ型胶原酶,建立脑出血模型,造模成功后6 h,移植组通过股静脉注射同种异体骨髓单个核细胞3×1010 L-1,模型组注射等量PBS。造模成功后1,4,8,16 d,评估大鼠神经功能;移植后16 d,观察大鼠脑损伤部位的病理变化;移植2,4 d后,采用流式细胞仪检测大鼠血肿脑组织内神经元细胞凋亡率。结果与结论:1神经功能:移植组8,16 d的神经功能缺损评分低于模型组(P<0.05)。2脑组织病理变化:正常对照组脑组织各层细胞排列紧密、结构完整,神经细胞和胶质细胞形态正常;模型组大鼠脑组织血肿周围组织疏松,细胞间出现间隙,出血部位神经细胞、胶质细胞大部分坏死;移植组大鼠脑组织细胞各层排列交紧密,较整齐,可见增生胶质细胞。3神经元细胞凋亡率:移植组血肿脑组织内细胞凋亡率低于模型组(P<0.05)。4结果表明,骨髓单个核细胞可明显促进脑出血后大鼠神经功能的恢复,降低大鼠脑组织内神经细胞凋亡。BACKGROUND： It has been proved that bone marrow mononuclear cell transplantation can obviously improve neurological function of rats with cerebral hemorrhage. OBJECTIVE： To investigate the effects of transplanted bone marrow mononuclear cells on the neurological function and apoptosis in perihematomal brain tissues following cerebral hemorrhage in a rat model. METHODS： Twenty-four Sprague-Dawley rats were given stereotaxical injection of collagenase IV into the caudate nucleus to establish cerebral hemorrhage models in transplantation group（n=12） and model group（n=12）, and then at 6 hours after cerebral hemorrhage, rats in these two groups were administrated 3x1010/L allograft bone marrow mononuclear cells and the same amount of PBS, respectively. Another 12 rats were given no interventions as control group. Neurological functions of rats were assessed at 1, 4, 8, 16 days after cerebral hemorrhage; pathological changes of the injury sites were observed at 16 days after transplantation; neuronal apoptosis rates in the perihematomal brain tissue were detected by flow cytometry at 2 and 4 days after transplantation. RESULTS AND CONCLUSION： The modified neurologic severity scores in the transplantation group were significantly lower than those in the model group at 8 and 16 days after cerebral hemorrhage（P〈0.05）. In the control group, cells in each layer arranged closely with complete structure, and neurons and glial cells were in good shape; in the model group, perihematomal brain tissues were loose with intercellular gap, in which most neurons and glial cells became necrotic; in the transplantation group, cells in each layer arranged closely and regularly, and glial cell proliferation occurred. Besides, compared with the model group, the neuronal apoptosis rate in the transplantation group was significantly lower（P〈0.05）. To conclude, bone marrow mononuclear cells can significantly enhance the neurological function recovery and reduce neuronal apoptosis in the brain of cerebral hemorrhag

关 键 词：干细胞 移植 脑出血 细胞凋亡 组织工程 骨髓单个核细胞 神经功能 

分 类 号：R394.2[医药卫生—医学遗传学]