神经干细胞移植治疗老年痴呆  

Neural stem cell transplantation for Alzheimer's disease

作　　者：张颖[1] 闫洪娟[1] 郭建华[1] 罗秋华[1] 李晓辉[2] 

机构地区：[1]邯郸市第一医院老年病二科,河北省邯郸市056000 [2]河北大学附属医院老年病科,河北省保定市071000

出　　处：《中国组织工程研究》2016年第19期2838-2843,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省医学科学研究重点课题(20130921)~~

摘　　要：背景:神经干细胞移植已应用于临床治疗脑瘫等脑损伤疾病,但对老年痴呆的相关研究并未深入。目的:观察神经干细胞移植对老年痴呆大鼠行为学及免疫调节系统的影响。方法:选取SD大鼠35只,切开脑后皮肤,在脑内海马区注射鹅膏蕈酸,建立老年痴呆模型;另取SD大鼠10只,切开脑后皮肤,在脑内海马区注射生理盐水,作为假手术组。将造模成功的32只老年痴呆大鼠随机分为2组,每组16只,实验组于脑内海马区注射5×109 L-1的同种异体神经干细胞悬液;模型组仅穿刺,不注射任何物质,移植后4周,对3组大鼠进行Morris水迷宫实验,连续5 d;水迷宫实验1周后,检测3组大鼠脑组织匀浆白细胞介素1、白细胞介素10水平,观察脑组织病理形态学变化。结果与结论:1Morris水迷宫实验:模型组认知和记忆能力均明显低于假手术组(P<0.01),实验组空间学习能力、记忆能力均显著高于模型组(P<0.05,P<0.01)。2脑组织匀浆炎炎性因子:模型组白细胞介素1、白细胞介素10水平明显高于假手术组(P<0.01),实验组白细胞介素1、白细胞介素10水平明显低于模型组(P<0.01)。3脑组织病理:模型组神经元细胞明显少于假手术组,实验组神经细胞数目明显多于模型组。4结果表明,神经干细胞移植对老年性痴呆大鼠神经元具有补充和保护作用,可明显改善动物的学习和记忆能力。BACKGROUND： Neural stem cell transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer＇s disease is rarely reported. OBJECTIVE： To observe the effect of neural stem cell transplantation on the behavior and immune regulating system of Alzheimer＇s disease rats. METHODS： Thirty-five Sprague-Dawley rats were enrolled to make a postcerebral incision and given hippocampal injection of amanita phalloides acid to establish rat models of Alzheimer＇s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups（n=16 per group）： rats in experimental group were administrated hippocamal injection of 5×10^9/L allogeneic neural stem cell suspension; those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as well as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION： Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group（P〈0.01）, and the abilities of spatial learning and memory were significantly higher in the experimental group（P〈0.05, P〈0.01）. Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group（P〈0.01） but significantly lower than those in the experimental group（P〈0.01）. Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer＇s disease in rats, thereby significantly improving the learning and memory abi

关 键 词：干细胞 神经干细胞 阿尔茨海默病 痴呆 组织工程 移植 老年痴呆 动物研究 干细胞移植 

分 类 号：R394.2[医药卫生—医学遗传学]