角膜缘干细胞缺乏是否可导致归巢因子基质细胞衍生因子1水平变化  

Variation of stromal cell-derived factor-1 level in a limbal stem cell deficiency model

作　　者：张伟[1] 黄文志[1] 张玉平[2] 

机构地区：[1]广州市第一人民医院老年病科眼科,广东省广州市510180 [2]广州市第一人民医院血液病科,广东省广州市510180

出　　处：《中国组织工程研究》2016年第19期2844-2849,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学青年科学基金项目(81300733)~~

摘　　要：背景:如何提高细胞移植的效率,促进来源有限、数量稀缺的角膜缘干细胞走向组织修复的第一步即归巢,是提高移植效率的关键。在视网膜色素上皮细胞的损伤修复中基质细胞衍生因子1可发挥着重要作用。目的:观察大鼠及小鼠眼局部角膜缘干细胞缺乏是否会引起外周血中归巢因子基质细胞衍生因子1质量浓度的变化,以期明确角膜缘干细胞缺乏是否会启动外周血中该因子产生变化,从而吸引外周血干细胞向角膜方向归巢。方法:剖切法制作大鼠和小鼠角膜缘干细胞缺乏模型眼,在为期1个月的观察期内连续抽取外周血,ELISA法检测基质细胞衍生因子1质量浓度。结果与结论:1大鼠外周血基质细胞衍生因子1质量浓度:在建模后24 h即发生变化,呈增长态势,在建模后第3天达到增长峰值,其后逐渐下降,但在第25天又出现1个增长峰值,较第3天的峰值低,其后恢复至正常数值;2小鼠外周血中基质细胞衍生因子1质量浓度:在建模后12 h即发生变化,呈增长态势,在建模后第3天达到增长峰值,其后逐渐下降,但在第20天又出现1个增长峰值,较第3天的峰值低,其后恢复至正常数值;3结果说明:外周血中基质细胞衍生因子1水平与角膜缘干细胞缺乏造成的损伤刺激存在一定关联,可能在干细胞迁移修复角膜损伤中起重要的作用。BACKGROUND： How to promote homing of scarce limbal stem cells is the key to improving transplantation efficiency. And stromal cell-derived factor-1 plays an important role in the repair of retinal pigment epithelial cells injury. OBJECTIVE： To observe changes of stromal cell-derived factor-1 levels in rat and mouse limbal stem cell deficiency models in order to confirm whether these changes will induce peripheral blood stem cells homing into the cornea. METHODS： Eye models of limbal stem cell deficiency were prepared in rats and mice by cutting method, then peripheral blood samples were continuously drawn to detect the mass concentration of stromal cell-derived factor-1 by enzyme-linked immunosorbent assay within 1 month after modeling. RESULTS AND CONCLUSION： In the rat peripheral blood, the mass concentration of stromal cell-derived factor-1 started to increase at 24 hours after modeling and firstly peaked at the 3rd day; then it tended to decrease and reached a second peak lower than the first one at the 25 th day; subsequently, it returned to the normal level. In the meanwhile, the mass concentration of stromal cell-derived factor-1 in the mouse peripheral blood, increased at 12 hours after modeling, peaked at the 3rd day, subsequently decreased until another peak lower than the former one presented at the 20 th day, and finally it returned to the normal level. In conclusion, the level of stromal cell-derived factor-1 in the peripheral blood is significantly associated with limbal stem cells deficiency, which may play an important role in corneal injury repair.

关 键 词：干细胞 组织工程 角膜 分化 角膜缘干细胞 角膜缘干细胞缺乏 外周血 基质细胞衍生因子1 归巢因子 ELISA 大鼠 小鼠 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]