机械因素对组织工程干细胞增殖和分化的影响  

Effects of mechanical factors on proliferation and differentiation of tissueengineered stem cells

作　　者：姜东[1] 王继宏[2] 

机构地区：[1]内蒙古医科大学,内蒙古自治区呼和浩特市010030 [2]内蒙古医科大学第二附属医院手足显微外科,内蒙古自治区呼和浩特市010030

出　　处：《中国组织工程研究》2016年第19期2864-2871,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81441117);课题名称:持续牵张应力刺激对兔跟腱损伤修复早期生物力学及相关因子表达的影响~~

摘　　要：背景:随着组织工程技术的发展和再生肌腱研究的深入,将解决传统肌腱移植所引起的诸多问题。目的:依据肌腱自身性质、干细胞的优缺点、机械力学系统的特点以及相应的力学系统对种子细胞的影响进行评价与展望。方法:检索中国生物医学文献数据库(CBM)、中国知识资源总库(CNKI)系列数据库、中文科技期刊数据库、Pub Med数据库2005年1月至2015年12月收录的肌腱组织工程相关综述和论文报告,检索词为组织工程(tissue engineering),肌腱(tendon),肌腱干细胞(tendon stem cell),力学刺激(mechanical stimulation),种子细胞(seeding cells),最终纳入63篇文献进行分析。结果与结论:1各种干细胞及相应的力学系统在组织工程的应用中各有优势和不足,研究者可根据研究需要加以选择;2肌腱干细胞在再生肌腱领域里应用有着广阔的前景,通过合适、精确、温和的机械力学刺激将肌腱干细胞培养成为满足需要的肌腱组织,将为医生和患者改善肌腱损伤后临床愈合效果提供另一种选择。BACKGROUND： With the development of tissue engineering technology and the deep research of tendon regeneration, many problems caused by traditional tendon transplantation will be solved. OBJECTIVE： To evaluate and prospect the effects of corresponding mechanical systems on seeding cells based on the characters of tendon, stem cells as well as mechanical systems. METHODS： CBM, CNKI, Cq Vip and Pub Med databases were retrieved for reviews and articles related to tissue-engineered tendon published from January in 2005 to December in 2015. The keywords were ＂tissue engineering, tendon, tendon stem cell, mechanical stimulation and seeding cells＂ in Chinese and English, respectively. Finally a total of 63 articles were selected for overview. RESULTS AND CONCLUSION： As all kinds of stem cells and the corresponding mechanical systems exhibit advantages and disadvantages when applied for tissue engineering, it is advisable to make appropriate choices according to the research needs. Tendon stem cells show a broad application prospect in tendon regeneration, which will grow into required tendon tissues through appropriate, accurate and gentle mechanical stimulations, thereby providing another alternative to improve the tendon healing.

关 键 词：干细胞 力学 细胞增殖 细胞分化 组织工程 分化 肌腱干细胞 种子细胞 体外培养 力学刺激 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]