急性髓细胞白血病患儿骨髓自分泌运动因子受体表达及其预后意义  

Expression of autocrine motility factor receptor in children with acute myelogenous leukemia and its correlation with prognosis

作　　者：王颖超[1] 朱桂英[1] 刘满菊 马丽娜[1] 刘东杰[1] 

机构地区：[1]郑州大学第一附属医院儿科,河南郑州450052

出　　处：《中华肿瘤防治杂志》2016年第5期304-307,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的糖蛋白AMFR（autocrine motility factor receptor）是自分泌运动因子（autocrine motility factor，AMF）的天然受体，AMFR与AMF结合后能促使细胞的运动、迁移。通过对急性髓细胞白血病（acute myelocytic leukemia，AML）患儿骨髓液单个核细胞AMFR表达情况的检测，探讨AMFR对患者预后的影响。方法选取2011-12-01-2013-12-31郑州大学第一附属医院儿科住院的初治核型正常的AML35例患儿作为初治组，选取同期住院排除恶性疾病的儿童30例作为对照组；采用实时定量PCR、蛋白质印记法检测初治组与对照组儿童骨髓单个核细胞中AMFR mRNA和蛋白的相对表达量，按AMFR mRNA二分位切点将初治组分为高表达组和低表达组，Kaplan-Meier法分析两组2年的总生存时间；Cox回归模型单因素和多因素分析AMFR表达水平、年龄、性别、外周血白细胞数目、骨髓原始细胞比例等因素与预后的相关性。结果初治组按标准化疗方案进行规律化疗，根据治疗效果可分为缓解组（n=19）与复发组（n=16）。初治组、缓解组、复发组与对照组AMFR mRNA的相对表达量分别为（0．61±0．11）、（0．38±0．07）、（0．86±0．12）与（0．19±0．02），各组之间两两比较，差异均有统计学意义，P〈0．001。初治组AMFR蛋白的相对表达量为（0．55±0．12），缓解组为0．28±0．07，复发组为0．72±0．13，对照组为0．14±0．06，各组之间两两比较，差异均有统计学意义，P〈0．001。采用Kaplan-Meier分析，AMFR低表达组中位生存期为710d（95％CI为116．409～245．251），高于AMFR高表达组的69d（95％CI为14．424～123．576），经Log-Rank统计分析，两组之间的总体生存率（OS）差异有统计学意义，P=0．003。采用Cox回归模型纠正性别、年龄、外周血白细胞数、骨髓原始细胞比例和白血病FAB分型等因素，AMFR基因mRNA表达水平和白细胞数是影响AML不良预后的�OBJECTIVE Autocrine motility factor receptor is a glycoprotein, which can promote cell moving and migrating by binding to autocrine motility factor. We can suppose that it is probably a prognostic factor by testing the expression of autocrine motility factor receptor in children with acute myelogenous leukemia. METHODS Form 2011-12-01 to 2013-12-31,35 children with acute myelogenous leukemia （ except acute promyelocytic leukemia） in the First Affiliated Hospital of Zhengzhou University Pediatric were enrolled in this research,while 30 healthy children were enrolled at the same time. We detected the autocrine motility factor receptor Messenger RNA（mRNA） and protein expression in the bone marrow mononuclear cells by Real-time quantitative-polymerase chain reaction and Western-blot respectively. Then we analyzed the 2 years overall survival time of AMFR-mRNA high expression and low expression group with the Kaplan Meier method and we used Cox regression model to correct and analysis factors which include gender, age, peripheral blood leukocyte counts and the proportion of bone marrow primitive cells as well as the FAB type of leukemia. RESULTS After regular chemotherapy, the children with AML could be divided into complete remission group（n=19） and recurrence group（n= 16）. The expressions of AMFR mRNA of the initial group, complete remission group, recurrence group and the control group were （0.61±0.11） ,（0.38±0.07） ,（0.86±0.12） and （0.19±0.02）respectively. Comparisons among these groups were statistically significant （P〈0. 001）. The expression of AMFR protein of the initial group, complete remission group, recurrence group and the control group were（0. 55±0.12）, （0.28±0.07）, （0.72±0.13） and （0.14±0.06） respectively. The comparisons among them were statistically significant too （P〈0. 001）. The median survival period of AMFR high expression group and low expression group of were 710 days （95%CI：116. 409-245. 251） and 69 days （95%CI： 14. 424

关 键 词：急性髓细胞白血病 自分泌运动因子 自分泌运动因子受体 预后 

分 类 号：R733.71[医药卫生—肿瘤]