HSV-TK基因重组腺病毒旁杀伤效应机制初探  

Study on effect of bystander effect of AdTK

作　　者：李秋香[1] 李冬田[1] 朱乃军[1] 佟惠春[1] 

机构地区：[1]天津医科大学微生物学教研室,天津300070

出　　处：《天津医科大学学报》2002年第3期294-296,共3页Journal of Tianjin Medical University

基　　金：天津市卫生局科技基金项目(99KY047)

摘　　要：目的 :探讨HSV -TK基因重组腺病毒 (AdTK)旁杀伤效应的机制。方法 :用MTT法计算细胞抑制率 ,数据采用单因素方差分析。结果 :病毒感染过的肿瘤细胞和经AdTK/GCV系统处理的肿瘤细胞培养上清液与未感染的肿瘤细胞按不同比例混合均显示出对肿瘤细胞的抑制作用。前者的抑制率随感染AdTK细胞比例的增加而增高 ,后者随上清液浓度的增加而升高。结论 :旁杀伤效应在TK自杀基因的治疗中起非常重要的作用。Objective: Bystander effect of AdTK was observed.Methods: Using MTT method the tumor cell growth inhibition rate of AdTK was measured.Results:The killing result from the two methods revealed that the growth inhibition rate increased with the raising of the ratio of TK-positive cell as well as the concentration of the supernatant from tumor cell treated with AdTK system.Conclusion: Bystander effect is very important in gene therapy of AdTK and it depends on co-effect of most link.

关 键 词：细胞抑制率 恶性肿瘤 自杀基因疗法 HSV-TK基因 重组腺病毒 旁杀伤效应 

分 类 号：R730.59[医药卫生—肿瘤]