siRNA下调SNCG基因表达抑制膀胱癌细胞系5637的增殖和侵袭  

siRNA-mediated downregulation of SNCG inhibits proliferation and invasiveness of bladder cancer cell line 5637

作　　者：陈志刚[1] 纪志刚[1] 石冰冰[1] 严维刚[1] 樊华[1] 李汉忠[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院泌尿外科,北京100730

出　　处：《基础医学与临床》2016年第6期835-839,共5页Basic and Clinical Medicine

摘　　要：目的探讨突触核蛋白γ(SNCG)基因对膀胱癌细胞5637增殖和侵袭能力的影响。方法设计并合成3对SNCG-siRNA序列,转染5637细胞后,用RT-q PCR和Western blot检测SNCG的表达。分别通过CCK-8增殖实验和侵袭实验评估5637细胞增殖和侵袭能力。结果与膀胱癌细胞系T24、EJ和UMUC-3相比,5637细胞中SNCG表达水平最高(P<0.05);与空白和阴性对照组相比,3对SNCG-siRNA序列转染5637后均可抑制SNCG mRNA和SNCG蛋白的表达(P<0.05),但SNCG-siRNA-244组抑制效果最明显;实验组5637细胞的增殖受到明显抑制(P<0.05),且细胞的侵袭能力显著下降(P<0.05)。结论通过特异性干扰SNCG基因的表达可抑制膀胱癌细胞5637的增殖和侵袭,SNCG的siRNA序列可能成为膀胱癌治疗的新靶点。Objective To explore the effects of γ-synuclein（ SNCG） on the proliferation and invasiveness of5637 bladder cancer cells. Methods Three pairs of SNCG-specific siRNAs were designed and transfected into the 5637 cell line,then the SNCG expression of the three siRNAs was assessed using RT-PCR and Western blot analysis,while the cell proliferation and invasiveness of the 5637 cells were evaluated by cell counting kit-8（ CCK-8） assay and transwell assay respectively. Results The bladder cancer cell line 5637 was used in next step（ P〈0. 05）. Compared with NC and empty vector controls,all three SNCG siRNAs were observed to significantly inhibit SNCG expression（ P〈0. 05）,among which the lowest SNCG expression was detected in SNCG-siRNA-244. The proliferation and invasiveness of 5637 cell line were both suppressed（ P〈0. 05）.Conclusions The proliferation and invasiveness of the bladder cancer cells may be decreased by specifically interferring the SNCG expression and SNCG siRNA may be a potential target for biomedical therapy in bladder cancer.

关 键 词：突触核蛋白γ 膀胱癌 5637细胞系 RNA干扰 

分 类 号：R737.14[医药卫生—肿瘤]