恶性纤维组织细胞瘤中肿瘤睾丸抗原相关基因的表达  

Expression of Cancer / testis Antigen-related Genes in Malignant Fibrous Histiocytoma

作　　者：董志伟[1] 王健[1] 穆苍山[1] 谷文光[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,黑龙江哈尔滨150001

出　　处：《实用骨科杂志》2016年第5期425-427,472,共4页Journal of Practical Orthopaedics

基　　金：黑龙江省博士后科研启动基金(LBH-Q12041)

摘　　要：目的检测肿瘤睾丸抗原相关基因在恶性纤维组织细胞瘤中的表达,探讨其作为靶抗原用于恶性纤维组织细胞瘤特异性免疫治疗的可能性。方法收集2010年4月至2015年1月确诊为恶性纤维组织瘤并行手术治疗的27例患者的肿瘤组织及癌旁组织。其中男17例,女10例;年龄31~83岁,平均（58.7±13.5）岁;临床分期：Ⅰ期5例,Ⅱ期7例,Ⅲ期11例,Ⅳ期4例;组织学分型：多形性14例,巨细胞性8例,炎性5例。用逆转录-聚合酶链反应方法对其肿瘤睾丸抗原相关基因（WT1,MPP11,PRAME,RHAMM,NY-CO-38,G250,NY-ESO-1,HTERT,BAGE）的表达进行检测。结果 27例恶性纤维组织瘤中肿瘤睾丸抗原相关基因的表达频率分别为WT1 77.8%（21/27）,MPP11 74.1%（20/27）,PRAME 63.0%（17/27）,RHAMM 59.3%（16/27）,NY-CO-38 51.9%（14/27）,G250 44.4%（12/27）,NY-ESO-1 44.4%（12/27）,HTERT 40.7%（11/27）,BAGE 14.8%（4/27）。9种肿瘤睾丸抗原相关基因在正常纤维组织中均无表达。肿瘤睾丸抗原相关基因在恶性纤维组织瘤中表达率最高的WT1与临床相关指标（性别、年龄、组织学亚型、临床分期）无显著相关性（P〉0.05）。结论 WT1等在恶性纤维组织瘤中呈现高表达,作为主动免疫治疗中的攻击靶点,具有很大的潜力。Objective To detect the expression of cancer / testis antigen related genes in malignant fibrous histiocytoma and to investigate the possibility of applying the antigens as the target antigens for malignant fibrous histiocytoma specific immunotherapy. Methods Tumor tissues and adjacent noncancerous tissues were collected in 27 patients diagnosed as malignant fibrous histiocytoma and received operation from April,2010 to January,2015. Among them,there were 17 male and 10 female with the average age（ 58. 7 ± 13. 5） years old（ 31 ~ 83 years old）. According to staging,5 cases were staged in Ⅰ,7 in Ⅱ,11 in Ⅲ,4 in Ⅳ; 14 cases were of pleomorphic,8 cases of giant cell and 5 cases of inflammatory out of 27 cases. Reverse-transcription polymerase chain reaction（ RT-PCR） was adopted to investigate the expression of cancer-testis antigen related genes（ WT1,MPP11,PRAME,RHAMM,NY-CO-38,G250,NY-ESO-1,HTERT,BAGE） of the tumor tissues and adjacent noncancerous tissues. Results The expression rates of cancer-testis antigen related genes in the 27 cases of malignant fibrous histiocytoma were WT1 77. 8%（ 21 /27）,MPP11 74. 1%（ 20 /27）,PRAME 63. 0%（ 17 /27）,RHAMM 59. 3%（ 16 /27）,NYCO-38 51. 9%（ 14 /27）,G250 44. 4%（ 12 /27）,NY-ESO-1 44. 4%（ 12 /27）,HTERT 40. 7%（ 11 /27）,BAGE 14. 8%（ 4 /27） respectively; almost none of the adjacent noncancerous tissues were positive for these genes. There was no significant correlation（ P〈0. 05） between cancer-testis antigen related genes WT1 with the highest expression rate in malignant fibrous histiocytoma and clinical correlation index（ gender,age,histologic subtypes,clinical stages）. Conclusion The cancer-testis antigen related genes（ WT1 et al） which represent high expression in malignant fibrous histiocytoma are suitable and potential attack target for the active immunotherapy of malignant fibrous histiocytoma.

关 键 词：恶性纤维组织细胞瘤 肿瘤睾丸抗原 肿瘤疫苗 免疫治疗 

分 类 号：R73-3[医药卫生—肿瘤]