TRAIL及其受体在子宫内膜腺癌细胞中的表达变化  

Changes of expressions of TRAIL and TRAIL receptor in endometrial cancer cells

作　　者：李晓兰[1] 谢环[1] 刘云[1] 

机构地区：[1]三峡大学第二人民医院,湖北宜昌434000

出　　处：《中国妇幼保健》2016年第11期2378-2381,共4页Maternal and Child Health Care of China

基　　金：湖北省自然科学基金面上项目(2015CFB574);湖北宜昌自然科学基金(A11301-39)

摘　　要：目的探讨TRAIL/TRAILR系统在子宫内膜癌细胞凋亡中的作用。方法选择正常子宫内膜组织(对照组)、早期子宫内膜腺癌组织(EEC组)、晚期子宫内膜腺癌组织(AEC组)各30例,各组织分别进行免疫组化、蛋白质印迹法检测TRAIL及其受体蛋白质表达量的变化和表达部位。结果 3组中TRAIL表达量无统计学差异(P>0.05);AEC组较对照组、EEC组DR4、DR5较对照组明显减少(P<0.01),EEC组DR4、DR5明显增加(P<0.01);EEC组DcR1、DcR2与对照组比较无统计学差异(P>0.05),AEC组较对照组及EEC组明显增加(P<0.01);EEC组OPG与对照组比较无统计学差异(P>0.05);AEC组较对照组及EEC组明显减少(P<0.01)。TRAIL及受体阳性表达主要定位表达于子宫内膜腺上皮细胞、血管平滑肌内皮细胞的细胞膜和胞浆等部位。结论 TRAIL在正常子宫内膜、早期子宫内膜腺癌及晚期子宫内膜腺癌发展过程中的表达无明显变化;子宫内膜腺癌早期死亡受体DR4,DR5表达明显减少,诱骗受体无显著变化,子宫内膜腺癌细胞凋亡过程中可能得以免疫耐受与被保护,引起子宫内膜腺癌细胞的凋亡逃逸;在子宫内膜腺癌的晚期阶段,子宫内膜腺癌细胞的死亡受体DR4,DR5,诱骗受体DcR1,DcR2的表达均明显增加,OPG的表达显著减少,TRAIL/TRAILR系统显著失衡,子宫内膜腺癌细胞生长迅速,易发生转移。OPG与子宫内膜腺癌细胞的凋亡逃逸是否有关尚需进一步研究。Objective To explore the role of tumor necrosis factor related apoptosis inducing ligand （TRAIL） and its receptor （TRAIL/TRAILR） system in apoptosis of endometrial cancer cells. Methods Thirty specimens of normal endometrial tissue （control group）, thirty specimens of early endometrial cancer tissue （EEC group）, and thirty specimens of advanced endometrial cancer tissue （AEC group） were selected, then immunohistochemieal staining and West blot were used to detect the changes and expression localizations of TRAIL and its receptor. Results There was no statistically significant difference in the expression level of TRAIL among the three groups （P〉0. 05 ） ; DR4 and DR5 levels in AEC group and EEC group were statistically significantly lower than those in control group （P〈0. 01 ）, DR4 and DR5 levels in EEC group increased significantly （ P〈0. 01 ） ; there was no statistically significant difference in DeR1 and DcR2 levels between EEC group and control group （P〉0. 05 ） , DcR1 and DcR2 levels in AEC group were statistically significantly higher than those in EEC group and control group （ P〈0. 01 ） ; there was no statistically significant difference in OPG level between EEC group and control group （P〉0. 05） ; OPG level in AEC group was statistically significantly lower than those in EEC group and control group （P〈0. 01 ） . TRAIL and TRAIL receptor mainly expressed in cell membrane and cytoplasm of endometrial glandular epithelial cells and vascular smooth muscle cells. Conclusion The expressions of TRAIL in normal endometrinm, early endometrial adenocarcinoma, and advanced endometrial adenocarcinoma don＇t change significantly; the expressions of endometrial adenocarcinoma early death receptors （ DR4 and DR5 ） decrease significantly, while the expressions of decoy receptors （DcR1 and DcR2 ） don＇t change significantly, which may be immune tolerance and protected in the process of endometrial glandular cells apoptosis, so apoptosis escape of endometr

关 键 词：子宫内膜癌 凋亡逃逸 TRAIL及其受体 

分 类 号：R737.33[医药卫生—肿瘤]