应用关联分析挖掘大豆对灰斑病12号生理小种的抗性位点  

Excavation the Resistance Locus to Cercospora sojina Hara Race 12 in Soybean by Association Analysis

作　　者：董亚楠[1,2] 魏崃[1] 丁俊杰[1] 王伟威[1] 于志远[1] 张丽[1] 杨晓杰[2] 刘丽君[1] 

机构地区：[1]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [2]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161000

出　　处：《大豆科学》2016年第3期402-406,共5页Soybean Science

基　　金：黑龙江省农业科技创新工程(2014ZD011);哈尔滨市应用技术与开发项目(2013RFQYJ016);国家现代农业产业技术体系(CARS-04-PS05)

摘　　要：利用126份SSR标记对320份对大豆灰斑病12号生理小种具有不同抗性的大豆材料进行基因组扫描、群体结构和连锁不平衡分析,并用STRUCTURE软件、TASSEL软件中的GLM分析方法对大豆抗性进行关联分析。结果表明:320份大豆品种中有23个表现高抗,53个表现抗病,178个表现中抗,56个感病,10个高感。找到与大豆抗灰斑病12号生理小种的相关位点共7个:H连锁群上的Satt052、F连锁群上的Satt335、C2连锁群上的Satt557、A1连锁群上的Sat_368、D1a连锁群上的Sat_346、O连锁群上的Satt243、J连锁群上的Sat_151。Genomic scanning,population structure and linkage disequilibrium analysis were carried out on 320 physiological races of soybean with different resistance to gray leaf spot disease 12 by using 126 SSR markers. Soybean resistance association analysis to Cercospora sojina Hara race 12 were run with software STRUCTURE and GLM procedure from software TASSEL. Research results showed that there were 23 soybean varieties showed high resistance,53 showed resistance to disease,178 were resistant,56 were susceptible,and 10 were high susceptible. Seven resistance locus to Cercospora sojina Hara race12 were found ineluding Satt052 on H linkage group,Satt335 on F linkage group,Satt557 on C2 linkage group,Sat_368 on A1 linkage group,Sat_346 on D1 a linkage group. Satt243 on O linkage group,Sat_151 on J linkage group.

关 键 词：大豆灰斑病菌 抗性位点 关联分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]