尖叶假龙胆不同提取部位对HepG2细胞胰岛素抵抗改善作用研究  

Effects of Different Fractions from Gmtianella Acuta on Improving Insulin Resistance in HepG2 Cells

作　　者：魏颖[1,2,3] 李蒙[1,2,3] 秦灵灵[2,3] 孙文[2,3] 李朋收[1,2,3] 徐暾海[1,2,3] 刘铜华[2,3] 

机构地区：[1]北京中医药大学中药学院,北京100029 [2]中医养生学北京市重点实验室,北京100029 [3]中医养生学教育部重点实验室,北京100029

出　　处：《中医药导报》2016年第12期13-16,20,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：科学技术部国家国际科技合作项目(2010DFB33260)

摘　　要：目的:研究尖叶假龙胆不同提取部位对正常HepG2细胞糖消耗及胰岛素抵抗HepG2细胞糖消耗的影响。方法:CCK-8法检测尖叶假龙胆不同提取部位对HepG2细胞增殖的影响;用葡萄糖氧化酶法检测尖叶假龙胆不同提取部位对HepG2细胞糖消耗的影响;用含有胰岛素的高糖培养基诱导HepG2细胞,建立胰岛素抵抗模型,并用葡萄糖氧化酶法检测尖叶假龙胆不同提取部位对胰岛素抵抗HepG2细胞糖消耗的影响。结果:浓度大于250μg/m L的各提取部位对细胞增殖均有明显抑制作用,除乙酸乙酯高浓度(250、200μg/m L)外其余提取部位对正常HepG2细胞的糖消耗均无显著影响。尖叶假龙胆乙酸乙酯部位、大孔树脂95%部位和大孔树脂70%部位均对胰岛素抵抗HepG2细胞糖消耗影响明显。结论:除乙酸乙酯高浓度(250、200μg/m L)外其余提取部位对正常HepG2细胞的糖消耗无显著影响。除大孔树脂30%部位外,其余各部位均能提高胰岛素抵抗的HepG2细胞的葡萄糖消耗,改善HepG2细胞的胰岛素抵抗。Objective： To study the effects of different fractions of Gmtianella Acuta on the glucose consumption of normal HepG2 cells and insulin-resistance HepG2 cell models. Methods： Proliferation of HepG2 cells was detected by CCk-8 assay. Detection of glucose consumption in HepG2 cells by using glucose oxidase method. Insulin resistance cell model was induced by high sugar medium （25 mmol/L） containing insulin. The influence of different extracts of Gmtianella Acuta on the glucose consumption of insulin-resistance HepG2 cell models were detected by glucose oxidase method. Results： The results showed that the different fractions from Gmtianella Acuta with concentration more than 250μg/mL have obvious inhibitory effect. There were no obvious influences on glucose consumption of normal HepG2 cells except ethyl acetate fraction with concentration of 250 and 200μg/mL. Influences of ethyl acetate fraction, macroporous resin-95% fraction and macroporous resin-70% fraction were remarkable on glucose consumption of insulin resistance HepG2 cell models. Conclusions： It was concluded that in addition to the high concentration of ethyl acetate （250 and 200 μg/mL）, the other rest fractions have no significant effect on glucose consumption of normal HepG2 cells. And different fractions from Gmtianella Acuta, except macroporous resin-30% fraction, could significantly promote the glucose consumption of insulin resistance in HepG2 cells and improve insulin resistance in HepG2 cells.

关 键 词：尖叶假龙胆 提取部位 HEPG2细胞 降糖活性 

分 类 号：R285.5[医药卫生—中药学]