自体骨髓间充质干细胞移植治疗神经系统疾病的临床研究  

Clinical Study of Treating Incurable Neurologic Diseases with Autologous Mesenchymal Stem Cells

作　　者：沈华超[1,2] 丁海霞[3] 李晓波[2] 曹菁菁[2] 马虎龙 张云云[2] 吴春平 孙波[2] 王杰[2,3] 丁新生[1,2,3] 

机构地区：[1]南京医科大学附属明基医院神经内科,南京210029 [2]南京医科大学明基神经病学研究所,南京210029 [3]南京医科大学第一附属医院神经内科,南京210029 [4]南京紫金医院内科,南京210007

出　　处：《神经损伤与功能重建》2016年第3期196-199,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.30971022)

摘　　要：目的:探讨应用自体骨髓间充质干细胞(MSCs)移植治疗神经系统疾病的临床疗效。方法:收集患者24例,包括持续植物状态(PVS)12例、运动神经元病11例、桥脑小脑萎缩1例。入院后经骨髓穿刺采集自体骨髓,当天分离回输1×108个单个核细胞,剩余细胞进行MSCs培养扩增,待MSCs数量增至约1×108个细胞后收集细胞,经腰椎穿刺推注。治疗前后对患者神经功能变化分别进行评价。结果:12例PVS患者中,PVS评分提高、肌力改善5例;11例运动神经元病患者和1例橄榄桥脑小脑萎缩患者中,肌力、构音障碍改善5例。结论:MSCs移植治疗可以部分缓解神经系统疾病的症状,近期疗效明显,无不良反应,远期疗效还需进一步随访和观察。Objective：To explore the clinical effects of auto-mesenchymal stem cells（MSCs） transplantation on treatment of patients with incurable neurologic diseases. Methods： The 24 patients with persistent vegetative state（n=12）, motor neuron disease（n=11） and olivoponto-cerebellar atrophy（n=1） were harvested for bone marrow. The mononuclear cells（MNCs） were isolated and 1×10^8MNCs were injected into subarachnoid space by lumbar puncture one the same day. The remaining cells were cultured, amplified and then injected into subarachnoid space by lumbar puncture when MSCs were increased to 1×10^8or so. The neurologic functions were observed before and after MSCs transplantation. Results： Five of 12 PVS patients had elevated PVS score at various degree and improved motor function. Among 11 motor neuron disease and 1 olivopontocerebellar atrophy patients, 5 patients had some improvement at various degrees in muscle strength, and speech. Conclusion：There were significant clinical effects in treatment of neurologic diseases with MSCs transplantation in a short term, and with no severe side effects and complications. The long-term clinical effect needs more observation with larger samples.

关 键 词：骨髓间充质干细胞 移植 持续植物状态 运动神经元病 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]