载脂蛋白E基因多态性与阿尔茨海默病的相关性研究  

Correlation between Apolipoprotein E Gene Polymorphisms and Alzheimer's Disease

作　　者：刘建强[1] 王晴[1] 井超[1] 

机构地区：[1]淄博市中心医院神经内科,山东淄博255036

出　　处：《神经损伤与功能重建》2016年第3期227-229,共3页Neural Injury and Functional Reconstruction

摘　　要：目的:探讨载脂蛋白E(Apo E)的多态性与阿尔茨海默病(AD)的相关性。方法:选取老年AD患者75例为AD组,另选取同期体检的健康老年人86例为对照组,采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)对2组的Apo E基因多态性进行鉴定,并分析其与AD患病的相关性。结果:AD组ε3/ε4基因型频率高于对照组,差异有统计学意义(χ2=4.27,P<0.05),ε3/ε3是最常见的基因型;AD组ε4等位基因频率高于对照组,差异有统计学意义(χ2=4.40,P<0.05),ε3是最常见的等位基因。相关危险度分析显示,Apo E的三个等位基因中Apo Eε4基因与AD有相关性(RR=3.76,P<0.05),Apoε2和Apoε3基因与AD无相关性(RR=0.64、0.32,P>0.05)。ε4基因为1或2个的患者AD患病率更高(χ2=3.87、4.62,P<0.05)。结论:Apo Eε4基因是AD患病的重要危险因素。Objective：To explore the correlation between the polymorphisms of apolipoprotein E（Apo E） and alzheimer＇s disease（AD）. Methods： Total 75 AD patients were enrolled as AD group, and 80 healthy old people were enrolled as control group. The polymorphisms of Apo E gene were identified with the method of polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Its correlation with AD was analyzed. Results： ε3/ε4 genotype frequency in the AD group was higher than that in the control group, with significant difference（χ^2=4.27, P〈0.05）. ε3/ε3 was the most common genotype. The frequency of ε4 alleles polymorphism in the AD group was higher than that in the control group, with significant difference（χ^2=4.40, P〈0.05）. ε3 alleles polymorphism was the most common. Related risk analysis showed that ε4 gene were associated with AD（RR =3.76, P〈0.05）, while ε3 and ε2 were not associated with AD（RR=0.64, 0.32, P〈0.05）. The prevalence of AD with one or two ε4 gene was higher（χ^2=3.87, 4.62, P〈0.05）. Conclusion：ε4 gene of the Apo E is an important risk factor for AD.

关 键 词：载脂蛋白E 阿尔茨海默病 基因多态性 相关性 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.021[医药卫生—临床医学]