三叶因子-2过表达对胃癌细胞株AGS增殖的影响  

Effect of TFF2 overexpression on proliferation of gastric cell line AGS

作　　者：司艺玲[1] 张金英[2] 赵亚力[3] 

机构地区：[1]解放军总医院基础所分子生物学研究室,北京100853 [2]解放军总医院基础所生物化学研究室,北京100853 [3]解放军总医院海南分院中心实验室,海南三亚572013

出　　处：《解放军医学院学报》2016年第6期621-624,共4页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81471052)~~

摘　　要：目的探讨三叶因子-2(trefoil factor family 2,TFF2)瞬时过表达对胃癌细胞增殖的影响及其机制。方法以真核表达质粒pcDNA3.1-Tff2转染AGS细胞,通过Western blot检测TFF2蛋白的表达,采用CCK-8检测细胞增殖能力的变化,Annexin V/PI法检测其凋亡。结果 pcDNA3.1-Tff2转染AGS细胞后TFF2蛋白表达水平较pc DNA3.1空质粒对照组升高5倍以上,pc DNA3.1-Tff2转染组AGS细胞增殖受到明显抑制,CCK-8实验96 hOD_(490nm)吸光度值较pc DNA3.1空质粒组显著下降(0.296 vs 2.108,P<0.01),凋亡率显著升高[(15.1%±1.21%)vs(5.49%±0.32%),P<0.01]。结论 TFF2在胃癌细胞中过表达可抑制其增殖并促进其凋亡,补充外源性TFF2可能成为一种有效抑制胃癌进展的治疗途径。Objective To study the effect of overexpressed Trefoil factor family 2（TFF2） on proliferation of human gastric carcinoma cell line AGS. Methods The recombinant plasmid pc DNA3.1- Tff2, including TFF2 c DNA, was transfected into AGS cells. Western blot was used to detect the expression of TFF2 protein. The effects of TFF2 on proliferation and apoptosis of AGS cells were analyzed using CCK-8 and Annexin V/PI. Results After successfully transfection of pc DNA3.1- Tff2, the expression of TFF2 was 5 times higher in protein level than that in empty plasmid group and control goup, and the proliferation of AGS cells in pc DNA3.1- Tff2 transfection group was inhibited significantly. Compared with empty plasmid group, CCK-8 assay showed that 96 h OD490nm absorbance in pc DNA3.1- Tff2 group decreased significantly（0.296 vs 2.108, P〈0.01）, while the percentage of cell apoptosis in pc DNA3.1-TFF2 group elevated significantly [（15.1%±1.21%） vs（5.49%±0.32%）, P 0.01]. Conclusion The overexpression of TFF2 can significantly decrease cell proliferation and promote apoptosis of AGS cell line, which may be an effective method in inhibiting the progress of gastric cancer.

关 键 词：三叶因子-2 胃癌细胞株AGS 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]