Slit2在非酒精性脂肪肝中的作用  

Effect of Slit2 on nonalcoholic fatty liver disease

作　　者：曾翠玲[1] 郑凌云 邢丽英[1] 李香莉[1] 王丽京[1] 

机构地区：[1]广东药学院,血管生物学研究所,广州510006

出　　处：《中国比较医学杂志》2016年第5期19-24,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(31271455;31200861)

摘　　要：目的探讨Slit2蛋白在非酒精性脂肪肝中的作用。方法选取8周龄雄性LDLR-/-小鼠和LDLR-/-;Slit2-Tg小鼠,各6只,高脂喂养12周建立NAFLD模型后,眼眶静脉丛取血测定血清TC,TG,LDL-C,HDL-C的水平,取肝脏组织做切片染色观察病理改变并提取肝组织RNA用QPCR分析LDLR-/-小鼠和LDLR-/-;Slit2-Tg小鼠脂代谢相关基因的表达水平。结果 LDLR-/-;Slit2-Tg小鼠血清中LDL-C水平明显低于LDLR-/-小鼠;与LDLR-/-小鼠相比,LDLR-/-;Slit2-Tg小鼠肝脏脂肪变性及肝损伤明显减轻,且肝脏组织脂代谢相关基因表达明显减少。结论 Slit2蛋白减轻了小鼠肝组织的病理损伤。Objective To investigate the effect of Slit2 over-expression on nonalcoholic fatty liver disease （NAFLD） in mice. Methods Eight week male LDLR-/- mouse and LDLR /- ;Slit2-Tg mouse were fed a high-fat diet for 12 weeks to induce NAFLD（n = 6 per group）. After 12 weeks, they were taken for blood samples and dissected. The concentration of TG （triglycerides）, TC（total cholesterol）, LDL-C（low density lipoprotein cholesterol） and HDL-C（ high density lipoprotein cholesterol） in the serum were examined respectively. Liver tissue samples were taken for HE staining to observe the morphological and pathological changes. The mRNA expression levels of hepatic genes related to lipid metabolism such as LXR-α,LXR-β FAS ,SREBP-lc was measured by real-time RT-PCR. Results The serum LDL-C levels of LDLR-/- ;Slit2-Tg mouse were significantly lower than that in LDLR-/- mouse（P 〈 0. 01 ）. And the grade of lipid droplets and pathological injury of liver tissue decreased significantly in the LDLR -/- ;Slit2-Tg mouse compared with LDLR-/- mouse, Furthermore, The mRNA expression levels of hepatic genes related to lipid metabolism decreased significantly in the LDLR /- ; Slit2-Tg mouse. Conclusions The overexpression of Slit2 can reduce the pathological injury of fiver tissue in mouse with NAFLD.

关 键 词：SLIT2 非酒精性脂肪肝 脂代谢 

分 类 号：R-332[医药卫生]