长爪沙鼠原位肝移植模型的建立  

Modified orthotopic liver transplantation in gerbils

作　　者：楼琦[1] 卢领群[1] 周冰珞 郭红刚[1] 李巍[1] 石巧娟[1] 杜江涛[1] 萨晓婴[1] 

机构地区：[1]浙江省医学科学院实验动物中心,浙江省实验动物与安全性研究重点实验室,杭州310010 [2]浙江大学医学院,杭州310053

出　　处：《中国比较医学杂志》2016年第5期53-57,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(31301933);浙江省科技计划项目(2013C37012;2013C37013;2014C27011)

摘　　要：目的建立长爪沙鼠原位肝移植模型的方法与技术。方法用改良的双套管法行100对长爪沙鼠原位肝移植手术,门静脉、肝下下腔静脉行套管吻合,而肝上上腔静脉采用缝合法,胆管内支架法完成胆道重建。结果在手术成熟后,供体手术时间为34.56±5.12 min,修肝及安置套管时间为15.43±2.75 min,受体手术时间为58.37±8.54 min,其中无肝期为23.66±4.47 min。手术成功率为86.67%(术后存活6 h以上)。结论改良的双套管法成功建立了长爪沙鼠肝移植模型,通过手术技巧训练可提高手术成功率,延长爪沙鼠存活时间。Objective To establish the orthotopic liver transplantation model in gebils. Methods Orthotopic liver transplantation was performed in 200 gerbils with modified two-cuff techniques. The suprahepatic vena cave was anastomosed end to end using nylon suture threat, the portal vein and the infrahepatic vena were inosculated by cuff method, respectively. Biliary tract reconstruction was completed in bile duct stent law. Result After paractise, donor surgery time was 34. 56 ±5.12 min, liver repaired time was 15.43 ±2. 75 min, surgery receptor time was 58.37 ± 8.54 min, no liver time was the success rate （survived more than 6 hours after operation） was 23.66 ±4. 47 min. Conclusion Orthotopic liver transplantation with modified two-cuff techniques in gerbils is a certain feasible. Improving operative techniques is of great importance in increasing successful rate and prolonging the survival time of gerbils after surgery.

关 键 词：长爪沙鼠 肝移植 模型 动物 

分 类 号：R-332[医药卫生]