TWS119对人NK细胞增殖和杀伤甲状腺癌BCPAP细胞的影响  

Effects of TWS119 on Human NK Cells Growth and Killing Function

作　　者：王海[1] 郑璐[2] 汤新星[1] 陈永强[2] 周忠海[2] 陈复兴[2] 

机构地区：[1]江苏大学附属人民医院核医学科,江苏镇江212002 [2]中国人民解放军第九七医院中心实验室,江苏徐州221004

出　　处：《标记免疫分析与临床》2016年第5期562-566,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨糖原合酶激酶-3β抑制剂TWS119对NK细胞增殖和杀伤功能的影响。方法从健康人外周血中分离单个核细胞（PBMCs）,加入到NK完全培养基培养,分别于第1d和第7d时,加入TWS119（0~8.0μmol/L）诱导培养。培养10 d后,用CCK-8法测定NK细胞增殖和杀伤甲状腺癌BCPAP细胞的能力;采用流式细胞术检测各组NK细胞纯度、颗粒酶和穿孔素的表达。结果培养10d后,第1天加入TWS119诱导组对NK细胞纯度和增殖呈现抑制作用;而第7天加入在一定浓度范围的TWS119诱导培养时能促进NK细胞的增殖和杀伤指标穿孔素的表达及对甲状腺癌BCPAP细胞的体外杀伤活性。结论 TWS119在一定浓度范围能促进γδT细胞增殖和增强对甲状腺癌BCPAP细胞的杀伤功能。Objective To investigate the effects of 4,6-disubstituted pyrrolopyrimidine（TWS119）,a glycogen synthase kinase-3β（GSK-3β） inhibitor on human NK cells growth and killing function.Methods Using SCGM medium to cultivate human peripheral blood NK cells in vitro.Different concentrations of TWS119 were added in the culture at day one and day 7,proliferation rates and killing activities of NK cells in each group were determined by CCK-8 and flowcytometry.Results After 10 days of culture,TWS119 inhibited the growth ratio of NK cells in day one group.In day 7 group,TWS119 could promote the growth of NK cells when the concentration was increased from 0 to 2.0 μmol / L and decreased thereafter.In addition,TWS119 at 0.5-8.0μmol / L could increase the expression of perforin and enhance the cytotoxic activity of NK cells to BCPAP cells.Conclusion TWS119 could promote the proliferation of NK cells and enhance the cytotoxic activity of NK cells to BCPAP cells.

关 键 词：NK细胞 TWS119 细胞增殖 BCPAP细胞 穿孔素 

分 类 号：R736.1[医药卫生—肿瘤]