犬乳牙牙髓干细胞体外培养及生物学性能研究  

Biological characteristics of stem cells from deciduous tooth pulp of Beagle dog

作　　者：刘飞[1] 刘阳[1] 戴姗姗[1] 马宁虎 张林[1] 郭青玉[1] 

机构地区：[1]陕西省牙颌面疾病临床研究中心.西安交通大学口腔医院儿童牙病科,710004

出　　处：《实用口腔医学杂志》2016年第3期331-335,共5页Journal of Practical Stomatology

基　　金：陕西省科学技术研究发展计划项目(编号:2013K12-16-04)

摘　　要：目的:体外分离培养比格犬乳牙牙髓干细胞(DTPSCs),研究其生物学特性。方法:用酶解组织块法培养6周龄比格犬DTPSCs,克隆法纯化,检测细胞克隆形成能力及增殖曲线;免疫组化鉴定细胞来源,流式细胞术鉴定细胞表面标记物;进行细胞多向诱导分化能力检测。结果:获得了成集落状生长的犬乳牙牙髓细胞,其克隆形成率为32%;免疫组化确定细胞为间充质而非造血来源;流式细胞术鉴定STRO-1、CD146阳性表达,而CD14、CD45及CD86阴性表达;细胞成骨、成脂诱导后分别对茜素红、油红O染色阳性,成牙本质诱导后细胞碱性磷酸酶、牙本质涎磷蛋白染色阳性,且细胞内碱性磷酸酶活性显著增高。结论:比格犬乳牙牙髓中存在具有间充质干细胞表型、自我更新及多向分化能力的干细胞。Objective： To culture and characterize the deciduous tooth pulp stem cells（ DTPSCs） of Beagle dog. Methods： DTPSCs were cultured using enzyme tissue block method from Beagle dog aged 6 weeks. The cells were purified by cloning culture,identified by immunohistochemistry and flowcytometry. The biological characteristics were studied by CCK-8 assay,osteogenetic induction,lipogenic induction and dentinogenic induction assays. Results： Beagle stem cells from deciduous tooth pulp were obtained,the cell colony formation rate was 32%. The cells were STRO-1 and CD146 positive,CD14,CD45 and CD86 negative. After multiple induction culture the cells were positive for alizarin red staining,oil red staining,ALP expression and DSSPP expression. Conclusion：The deciduous tooth pulp stem cells of Beagle dog have multilineage differentiation abilities.

关 键 词：犬 乳牙牙髓干细胞(DPSCs) 多向分化 

分 类 号：R781[医药卫生—口腔医学]