釉原蛋白对牙周膜干细胞迁移、黏附及增殖的影响  

The effects of amelogenin on the migration,adhesion and proliferation of periodontal ligament stem cells

作　　者：丰培勋[1] 王爽[2] 刘瑶[1] 曹沛[2] 李克翔[2] 张海娟[2] 

机构地区：[1]武警陕西总队医院口腔科,西安710054 [2]西安交通大学口腔医学院正畸科

出　　处：《实用口腔医学杂志》2016年第3期383-388,共6页Journal of Practical Stomatology

基　　金：武警陕西总队医院基础课题(编号:2013002);中央高校基本科研业务费专项资金(编号:2012jdhz27)

摘　　要：目的:检测釉原蛋白(amelogenin,AML)对牙周膜干细胞(PDLSCs)迁移、黏附和增殖的影响。方法:用0.25、50和100μg/ml的AML培养人PDLSCs。用划痕实验和transwell法检测AML对PDLSCs迁移的影响。用黏附实验检测AML对PDLSCs黏附的影响。用MTT法和计数法检测AML对PDLSCs增殖的影响。结果:AML可促进PDLSCs的迁移,而且呈剂量效应关系(P<0.05)。AML对PDLSCs迁移和黏附有促进作用(P<0.05),且随培养时间延长而增加。AML可促进PDLSCs的增殖,且呈剂量效应关系。结论:AML可促进PDLSCs的迁移、黏附和增殖。Objective： To evaluate the effects of amelogenin（ AML） on the migration,adhesion and proliferation of periodontal ligament stem cells（ PDLSCs）. Methods： PDLSCs were cultured with AML at 0. 25,50 amd 100 μg / ml respectively. The migration,adhesion and proliferation of the cells were examined by wound healing migration assay,transwell migration assay,attachment assay,MTT assay and cell counting,respectively. Results： AML induced the migration of PDLSCs in a dose-dependent manner（ P〈0. 05）,increased the adhesion and proliferation of PDLSCs（ P〈0. 05）. Conclusion： AML may promote the migration,adhesion and proliferation of PDLSCs.

关 键 词：牙周膜干细胞(PDLSCs) 釉原蛋白 迁移 黏附 增殖 

分 类 号：R781[医药卫生—口腔医学]