PPM1D mRNA表达与肝癌预后的相关性研究  

Relation of phosphatase magnesium-dependent 1δ (PPM1D) mRNA expression and hepatocellular carcinoma prognosis

作　　者：袁莉[1] 刘军[2] 李光兵[2] 

机构地区：[1]山东大学附属省立医院麻醉手术科,山东济南250021 [2]山东大学附属省立医院器官移植肝胆外二科,山东济南250021

出　　处：《中国现代普通外科进展》2016年第4期263-266,270,共5页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金面上项目(81373172)

摘　　要：目的 :探讨PPM1D m RNA表达与肝癌预后的相关性。方法:提取86例肝癌患者癌组织及癌旁肝组织总RNA,q PCR法检测PPM1D m RNA表达量,免疫组化检测蛋白表达水平。根据癌旁肝组织中PPM1D m RNA表达量,将肝癌患者分组为高表达组与低表达组,对两组患者临床资料及生存时间进行统计分析。结果:PPM1D m RNA在肝癌组织中表达水平显著高于癌旁组织,免疫组化检测蛋白表达水平证实上述结果。以癌旁肝组织PPM1D m RNA表达量为阈值,高表达组56例,低表达组30例。两组患者的AFP水平、肿瘤大小、肿瘤TNM分期以及肿瘤复发、家族史等临床病理因素差异有统计学意义(P<0.01);年龄、性别、门静脉侵犯、淋巴结转移、HBV感染及酒精摄入史等因素差异无统计学意义(P>0.05)。高表达组患者中位生存期为13个月,低表达组为32个月。结论:PPM1D m RNA表达水平可能与肝癌恶性程度相关,可能成为肝癌预后的预测因子。Objective： Protein phosphatase magnesium- dependent 1δ（PPM1D）is an oncogene, overexpressed in many solid tumors, including ovarian cancer and breast cancer. The current study examined the expression and the prognostic value of PPM1 D m RNA in human hepatocellular carcinoma（HCC）. Methods： Total RNA was extracted from 86 HCC and paired non- cancerous liver tissues. PPM1 D m RNA expression was determined by real- time quantitative reverse transcrip-tase- polymerase chain reaction（q PCR）. Immunohistochemistry assay was used to verify the expression of ppm1 d protein in the HCC and non- cancerous liver tissues. HCC patients were grouped according to PPM1 D m RNA expression with the average PPM1 D m RNA level in non- cancerous liver tissue samples as the cut- off. Correlations between clinicopathologic variables, overall survival and PPM1 D m RNA expression were analyzed. Results： PPM1 D m RNA was signi cantly higher in HCC than in the paired non- cancerous tissue（P〈0.01）. This was confirmed by ppm1 d staining. 56 patients were classified as high expression group and the other 30 patients were categorized as low expression group. There were significant differences between the two groups in term of alpha- fetoprotein（α- FP）level（P〈0.01）, tumor size（P〈0.01）, TNM stage（P〈0.01）, recurrence incidence（P〈0.01）and family history of liver cancer（P〈0.01）. The current study failed to find significant differences between the two groups in the following clinical characteristics： age, gender, portal vein invasion, lymphnode metastasis, hepatitis B virus（HBV）infection and alcohol intake. Survival time of high expression group was significantly shorter than that of low expression group（median survival, 13 months and 32 months,respectively, P〈0.01）. Conclusion： Up- regulation of PPM1 D m RNA was associated with progressive pathological feature and poor prognosis in HCCpatients. PPM1 D m RNA may serve as a prognostic marker in HCC.

关 键 词：肝肿瘤 PPM1D MRNA 预后 

分 类 号：R735.7[医药卫生—肿瘤]