磷脂酶C δ1(PLCD1)抑制CAPAN-1和BXPC-3胰腺癌细胞增殖和侵袭迁移  

Phospholipase C δ1(PLCD1) inhibits the proliferation,invasion and migration of CAPAN-1 and BXPC-3 pancreatic cancer cells

作　　者：胡丹[1] 姜政[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《细胞与分子免疫学杂志》2016年第6期739-745,共7页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨转染磷脂酶Cδ1(PLCD1)对胰腺癌细胞生物学行为的影响。方法培养HPDE6C7人正常胰腺导管上皮细胞和CAPAN-1、PANC-1、ASPC-1及BXPC-3胰腺癌细胞,反转录PCR筛选出低表达PLCD1的胰腺癌细胞;将挑选出的CAPAN-1和BXPC-3胰腺癌细胞转染pc DNA3.1-PLCD1,并设置空载体pc DNA3.1转染组。反转录PCR检测CAPAN-1和BXPC-3细胞PLCD1 mRNA的表达水平,Western blot法检测其PLCD1蛋白水平。CAPAN-1和BXPC-3细胞转染PLCD1后,CCK-8法检测细胞增殖活性,流式细胞术分析细胞周期和凋亡情况,Transwell^(TM)法检测细胞的侵袭和迁移能力。结果筛选出低表达PLCD1的CAPAN-1和BXPC-3细胞,转染pc DNA3.1-PLCD1的CAPAN-1和BXPC-3细胞PLCD1 mRNA和PLCD1蛋白水平明显增加;细胞增殖、侵袭和迁移受到抑制;细胞周期阻滞于G0/G1期,细胞凋亡增加。结论转入PLCD1促进CAPAN-1和BXPC-3胰腺癌细胞凋亡并使细胞周期阻滞于G0/G1期,同时抑制其增殖、侵袭和迁移。Objective To explore the effect of phospholipase C δ1（ PLCD1） transfection on the biological behaviors of pancreatic cancer cells. Methods HPDE6C7 normal human pancreatic ductal epithelial cells and CAPAN-1,PANC-1,ASPC-1and BXPC-3 pancreatic cancer cells were cultured. Reverse transcription PCR was used to screen the pancreatic cancer cells with low expression level of PLCD1. Recombinant plasmid pc DNA3. 1-PLCD1 transfected pancreatic cancer cells CAPAN-1and BXPC-3 with low expression level of PLCD1,and meanwhile the corresponding vector pc DNA3. 1 transfection group was set up. The expression level of PLCD1 mRNA was detected by reverse transcription PCR,and the protein level was examined by Western blotting in pancreatic cancer cells CAPAN-1 and BXPC-3 after transfection. After the expression of PLCD1 was upregulalted in CAPAN-1 and BXPC-3 pancreatic cancer cells,the cell proliferation was analyzed by CCK-8assay; flow cytometry was used to test the cell cycle and apoptosis; Transwell^（TM）assay was performed to evaluate the invasion and migration ability of the cells. Results The study obtained CAPAN-1 and BXPC-3 cells with lower expression of PLCD1. The expression levels of PLCD1 mRNA and protein obviously increased in CAPAN-1 and BXPC-3 cells after transfected with plasmid pc DNA3. 1-PLCD1,and the abilities of cell proliferation,invasion and migration were restrained. The cell cycle was arrested in G0/G1 phase,and cell apoptosis was remarkably promoted. Conclusion PLCD1 transfection can induce the apoptosis of CAPAN-1 and BXPC-3 cells,arrest the cell cycle in G0/G1 phase. Meanwhile,it inhibits the abilities of cell proliferation,invasion and migration of CAPAN-1 and BXPC-3 cells.

关 键 词：PLCD1 胰腺癌 周期 凋亡 迁移 侵袭 

分 类 号：R735.9[医药卫生—肿瘤] R576[医药卫生—临床医学]