集聚蛋白基因在不同宫颈癌细胞中的表达  

Expression of Agrin gene in different human cervical carcinoma cells

作　　者：冯思佳[1] 崔静[1] 杨璐[1] 赵二趁 柴婕[1] 杨丹丹[1] 张翠翠[1] 王志慧[1] 郜培琼 千新来[1] 

机构地区：[1]新乡医学院基础医学院病理学教研室,河南新乡453003

出　　处：《新乡医学院学报》2016年第5期365-368,共4页Journal of Xinxiang Medical University

基　　金：河南高校科技创新团队项目资助(编号:2012IRTSTHN015);河南省基础与前沿技术研究计划项目资助(编号:122300410121);河南省科技创新杰出青年基金资助项目(编号:094100510007);新乡医学院重点学科开放课题资助项目(编号:ZD200955)

摘　　要：目的探讨集聚蛋白(Agrin)基因在人乳头瘤病毒(HPV)阳性和阴性宫颈癌细胞中表达的差异。方法以人乳头瘤病毒16(HPV16)阳性的宫颈癌Caski细胞和Si Ha细胞、HPV18阳性的宫颈癌He La细胞、HPV阴性的宫颈癌C33-A细胞为研究对象。通过Western blot和实时荧光定量聚合酶链反应(PCR)等方法分别检测Agrin mRNA和蛋白在不同宫颈癌细胞中的表达。结果实时荧光定量PCR和Western blot结果均显示,与HPV阴性的宫颈癌C33-A细胞比较,HPV16阳性的宫颈癌Caski和Si Ha细胞、HPV18阳性的宫颈癌He La细胞中的Agrin mRNA和蛋白表达均显著增加(P<0.01或0.05),Agrin mRNA和蛋白在4种宫颈癌细胞中表达的顺序为:C33-A细胞<Si Ha细胞<Caski细胞和He La细胞。结论 HPV阳性的宫颈癌细胞中Agrin基因的表达显著高于HPV阴性的宫颈癌细胞,为深入研究Agrin基因与宫颈癌的关系及相关机制奠定了基础。Objective To investigate the expression of Agrin gene in human papillomavirus（ HPV）-positive and-negative cervical carcinoma cells. Methods Cervical carcinoma Caski cells（ HPV16-positive）,Si Ha cells（ HPV16-positive）,He La cells（ HPV18-positive） and C33-A cells（ HPV-negative） were taken as the research objects. Real-time fluorescence quantitative polymerase chain reaction（ PCR） and Western blot methods were used to detect the expressions of Agrin mRNA and protein in different cervical carcinoma cells. Results Real-time fluorescence quantitative PCR and Western blot results indicated that compared with C33-A cells（ HPV-negative）,the Agrin mRNA and protein expressions in Caski cells（ HPV16-positive）,Si Ha cells（ HPV16-positive） and He La cells（ HPV18-positive） were significantly increased（ P 0. 01 or 0. 05）. The order of Agrin mRNA and protein expressions in the four kinds of cervical carcinoma cells was as follows： C33-A cells Si Ha cells Caski cells and He La cells. Conclusion The expression of Agrin gene in HPV-positive cervical carcinoma cells is significantly higher than that in HPV-negative cervical carcinoma cells,which has laid a foundation for further research on the relationship between Agrin gene and cervical carcinoma and its mechanisms.

关 键 词：宫颈癌 集聚蛋白 信使RNA 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]