电针水沟穴对大脑中动脉闭塞大鼠脑缺血后微小RNA-126及靶基因的影响  

Effect of electro-acupuncture at Shuigou acupoint on expressions of microRNA-126 and target genes after cerebral ischemia in rats with middle cerebral artery oeclusion

作　　者：李莎莎[1] 刘津溪 钱小路[1] 靳兰洁 袁影[1] 周爽[1] 

机构地区：[1]第二军医大学长海医院中医系,上海200433

出　　处：《中国中西医结合急救杂志》2016年第3期232-235,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目（81173330,81574059）

摘　　要：目的观察电针水沟穴对大脑中动脉闭塞（MCAO）大鼠脑缺血后微小RNA-126（miR-126）及其靶基因磷脂酰肌醇-3-激酶调节亚单位（PIK3R2）mRNA、出芽相关蛋白-1（SPRED1）mRNA表达的影响，从血管新生角度探讨电针治疗脑缺血的可能机制。方法将20只雄性SD大鼠按随机数字表法分为正常组、假手术组、模型组、治疗组，每组5只。参照改良Longa线栓法复制MCAO大鼠模型，假手术组不插入线拴。制模后治疗组于水沟穴（大鼠唇裂鼻尖下1mm中处，向上斜剌1mm）与右耳根部皮肤接G6805—1A型治疗仪进行电针治疗，1mA电流、2Hz，持续留针30min，连续治疗3d；正常组、假手术组、模型组制模后不进行处理。采用Berderson评分评价大鼠神经功能缺损程度；用实时荧光定量聚合酶链反应（qPCR）检测miR-126及PIK3R2、SPRED1的mRNA表达。结果与正常组比较，模型组大鼠有不同程度的神经功能缺损，Berderson评分明显增高（分：11．20±1．64比0，P〈0．01），治疗后Berderson评分较模型组明屁降低（分：7．20±1．48比11．20±1．64，P〈0．01）。模型组miR-126、PIK3R2及SPRED1的mRNA表达均较正常组明显升高[miR-126（2^-△△Cr）：1．13±0．48比0．16±0．12．PIK3R2mRNA（2^-△△Cr）：12．03±6．09比0．42±0．33，SPRED1mRNA（2^-△△Cr）：8．77±3．85比0．21±0．40，均P〈0．01]；治疗组miR-126表达较模型组明显升高（2^-△△Cr：1．93±0．81比1．13±0．48），PIK3R2、SPRED1的mRNA表达较模型组明照明显降低[PIK3R2mRNA（2^-△△Cr）：5．78±3．61比12．03±6．09，SPRED1mRNA（2^-△△Cr）：3．90±3．24比8．77±3．85，P〈0．05或JP〈0．01]。结论电针水沟穴能促大鼠脑缺血后神经功能体征的恢复，其机制可能与其调控miR-126及其靶基因从而促进了脑血管新生有关.Objective To observe the influence of electro-aeupuncture （EA） at Shuigou （GV26） on the expressions of microRNA-126 （miR-126） and its target genes-phosphoinositol-3 kinase regulatory subunit 2 （PIK3R2）, spronty-related protein-1 （SPRED1） mRNA after cerebral ischemia in rat models with middle cerebral artery occlusion （MCAO） and discuss the possible mechanism of EA treatment for cerebral ischemia from the perspective of angiogenesis. Methods Twenty male Sprague-Dawley （SD） rats were randomly divided into four groups： normal, sham operation, model and treatment groups （5 rats in each group）. Modified Longa arterial thread embolism method was employed to establish the experimental rat models with intra-luminal MCAO, and in the sham operation group no thread embolism was inserted. In the treatment group, after model establishment EA was given at Shuigou （the acupoint is located at a point 1 mm below the nose tip in the median line between the nose and lip, and the direction of puncture is obliquely upward for 1 mm in depth）, and the G6805-1A type EA treatment instrument was connected at the root part skin of right ear with 1 mA current, 2 Hz, retaining the needle continuously for 30 minutes once daily for consecutive 3 days. Normal group, sham operation group, model group were not intervened. The neurological deficits in rats were evaluated by Berderson score. The expressions of miR-126 and PIK3R2, SPREDI mRNA in isehemic tissues were detected by fluorescent quantitative polymerase chain reaction （qPCR）. Results Compared with normal group, there were different degrees of neurological deficits and Berderson score was significantly higher in model group （11.20 ±1.64 vs. 0, P 〈 0.01）; after treatment, the Berderson score in treatment group was markedly lower compared with that in model group （7.20 ± 1.48 vs. 11.20 ±1.64, P 〈 0.01）. Compared with normal group, the expressions of miR-126 and PIK3R2, SPRED1 mRNA in ischemic tissues of model group were significantly h

关 键 词：脑缺血 电针 水沟穴 微小RNA-126 磷脂酰肌醇-3-激酶调节亚单位2 出芽相关蛋白-1 

分 类 号：R743.31[医药卫生—神经病学与精神病学]