MiR-200a对结直肠癌SW1116细胞侵袭和迁移的影响  

Effect of miR-200a on the migration and invasion of colorectal cancer SW1116 cells

作　　者：李懿君[1] 李昊[1] 尹玉[1] 孙晶璐 江燕[1] 詹鹤琴[1] 杨枫[1] 詹磊[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032

出　　处：《安徽医科大学学报》2016年第6期791-794,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81201536);安徽高校省级自然科学基金(编号:KJ2011A160)

摘　　要：目的探讨微小RNA(miR)-200a的过表达对结直肠癌SW1116细胞迁移和侵袭能力的影响。方法利用LipofectamineTM2000瞬时转染含miR-200a的质粒,实验组将载有miR-200a的质粒转染至结直肠癌SW1116细胞中,细胞对照组结直肠癌SW1116细胞不做任何处理;应用实时定量PCR法分析miR-200a的表达变化,划痕实验检测细胞迁移能力的改变、Transwell小室侵袭实验检测细胞侵袭能力的改变。结果实时定量PCR结果表明,与细胞对照组比较,实验组经瞬时转染后miR-200a的表达水平显著升高(P<0.01);划痕实验和Transwell小室侵袭实验表明外源性过表达miR-200a可促进结直肠癌SW1116细胞的迁移和侵袭能力,与细胞对照组比较差异均有统计学意义(P<0.01)。结论miR-200a能明显促进结直肠癌SW1116细胞的侵袭和迁移。Objective To investigate the effect of microRNA（ miR）-200 a on migration and invasion abilities of colorectal cancer SW1116 cells. Methods miR-200 a plasmid was transfected into SW1116 cells in colorectal cancer SW1116 cells by LipofectamineTM2000 as the experimental group,colorectal cancer SW1116 cells in the control group do not do any processing. The expression of miR-200 a in the experimental group was confirmed by real-time PCR. The migration abilities of cells were detected by Transwell invasion assay,and the invasion abilities of cells were detected by wound-healing assay. Results Real-time quantitative PCR results showed that compared with the control group,experimental group after transient transfection of miR-200 a was significantly increased（ P 〈0. 01）. Exogenous overexpression of miR-200 a could promote migration and invasion abilities of SW1116 cells,compared with the control group the difference was statistically significant（ P〈 0. 01）. Conclusion miR-200 a can promote the migration and invasion abilities of colorectal cancer SW1116 cells.

关 键 词：结直肠癌 miR-200a 侵袭 迁移 

分 类 号：R735.3[医药卫生—肿瘤]