原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响  

Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide

作　　者：陈金枝[1,2] 张小强[1,2] 曲志华[1,2] 周亚盼 张妍[1,2] 梁晓瑜[1,2] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]东南大学环境医学工程教育部重点实验室,江苏南京210009

出　　处：《癌变．畸变．突变》2016年第3期214-217,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：中央高校基本科研业务费专项资金资助;江苏省普通高校研究生科研创新计划资助项目资助(SJZZ_0032);江苏省卫生厅预防医学科研基金(Y2013070)

摘　　要：目的:探讨原花青素对脂多糖(LPS)激活小鼠小胶质细胞(BV2)炎症介质分泌的影响。方法:以LPS激活BV2细胞构建神经炎症模型。分别采用0.1、0.5、1.0、5.0μg/mL原花青素预处理后,1.0μg/mL的LPS刺激BV2细胞24 h。采用MTT法检测细胞存活率;Griess法检测BV2细胞培养液上清中一氧化氮(NO)水平;ELISA法检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的分泌水平。结果:在实验剂量范围内,原花青素及LPS对BV2细胞活性均无显著影响(P>0.05)。但1.0μg/mL LPS可引起BV2细胞各炎症因子NO、TNF-α、IL-1β和IL-6水平明显升高(P<0.05)。与LPS组相比,原花青素(0.1、0.5、1.0、5.0μg/mL)预处理能使激活的BV2细胞培养液上清中NO释放量减少(P<0.05),TNF-α、IL-1β和IL-6含量降低(P<0.05),抑制作用呈剂量一效应关系。结论:在体外实验中,原花青素对LPS诱导小胶质细胞所致的炎症反应具有保护作用。OBJECTIVE：To investigate the effects of pro-anthocyanidin on inhibiting inflammatory mediator secretion in LPS activation of BV2 microglia.METHODS：Neuroinflammation model was established by LPS-activated BV2 microglia.BV2 microglial were pretreated with different concentrations of pro-anthocyanidin（0.1,0.5,1.0,5.0μg/mL） and stimulated with LPS（1.0μg/mL） for 24 h.Cell viability was assessed by MTT assay.Griess method was utilized to detect the content of NO.The secretion level of pro-inflammatory cytokines TNF-alpha,IL-lbeta and IL-6 was examined using specific ELISA kits.RESULTS：Within a certain dosage ranges of pro-anthocyanidin and LPS,there was no negative effect on cell viability（P〉0.05）.LPS（1.0 μg/mL） significantly increased the release of inflammatory mediator nitric oxide（NO） as well as the cytokines TNF-α,IL-1β and IL-6（P〈0.05）.Compared with the LPS group,different concentrations of pro-anthocyanidin（0.1,0.5,1.0,5.0 μg/mL） attenuated the release of NO,TNF-α,IL-1β and IL-6 in cells that were cultured in conditioned media from LPS-induced BV2 microglia.The effect was dose-dependent（P〈0.05）.CONCLUSION：We showed that pro-anthocyanidin has a protective effect on inflammatory responses of LPS-activated BV2 microglia in vitro.

关 键 词：原花青素 BV2细胞 脂多糖 神经炎症 

分 类 号：R994.6[医药卫生—毒理学]