唾液腺非乳腺样分泌癌中乳腺球蛋白的表达及ETV6基因重排  

Mammaglobin expression and ETV6 gene alteration in salivary non-mammary analogue secretory carcinomas

作　　者：孙晶晶[1] 田臻[1] 王丽珍[1] 张春叶[1] 张莺[1] 李江[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔病理科,上海市口腔医学重点实验室,上海200011

出　　处：《中国口腔颌面外科杂志》2016年第3期223-227,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81372910);教育部博士点基金(20120073110084)

摘　　要：目的 :分析乳腺球蛋白(mammaglobin,MMG)在唾液腺非乳腺样分泌癌中是否存在表达,以及有表达者是否存在ETV6基因分离,以评价MMG及ETV6基因检测在唾液腺癌病理诊断中的价值。方法 :选取95例唾液腺非乳腺样分泌癌(包括20例腺泡细胞癌,10例多形性低度恶性腺癌,6例唾液腺导管癌,9例囊腺癌,6例低度恶性筛状囊腺癌,14例黏液表皮样癌,14例恶性多形性腺瘤,16例腺样囊性癌)行MMG免疫组织化学染色,利用荧光原位杂交(FISH)技术进一步分析阳性染色的病例。结果:29/95例病例出现MMG阳性表达,其中17例为MMG强阳性,包括4/6例唾液腺导管癌、4/6例低度恶性筛状囊腺癌、4/9例囊腺癌、3/8例恶性多形性腺瘤(腺癌)、1/10例多形性低度恶性腺癌、1/4例高度恶性黏液表皮样癌。全部29例MMG阳性肿瘤经FISH检测,均未发现ETV6基因分离。结论:MMG在部分非乳腺样分泌癌中阳性表达,尤其在唾液腺导管癌、低度恶性筛状囊腺癌、囊腺癌中有较高比例的强阳性表达,但MMG阳性表达的非乳腺样分泌癌均不存在ETV6基因分离,提示MMG在鉴别诊断中作用有限,必要时还需辅助分子检测。PURPOSE： To determine the diagnostic value of mammaglobin（MMG） expression and ETV6 gene rearrangement in salivary gland carcinomas in addition to mammary analogue secretory carcinomas（MASC）. METHODS：Ninety-five cases of salivary non-MASC carcinomas including 20 acinic cell carcinomas（Aci CC）, 10 polymorphous lowgrade adenocarcinomas（PLGA）, 6 salivary ductal carcinomas（SDC）, 9 cystadenocarcinomas（CAC）, 6 low-grade cribriform cystadenocarcinomas（LGCCC）, 14 mucoepidermoid carcinomas（MEC）, 14 malignant pleomorphic adenomas（MPA）, and16 adenoid cystic carcinomas（ACC） were selected for MMG immunohistochemical staining, and fluorescence in situ hybridization（FISH） method was further performed in the cases characterized as MMG positive. RESULTS： 29/95 cases showed diffuse or focal staining for MMG and 17 cases demonstrated strong positivity, which included 4/6 SDC, 4/9 CAC,4/6 LGCCC, 3/8 MPA（adenocarcinoma subtype）, 1/10 PLGA and 1/4 high grade MEC. ETV6 gene rearrangement was not detected in any of the 29 MMG-positive cases. CONCLUSIONS： MMG expression was observed in some cases of salivary non-MASC carcinomas, and a higher proportion of strongly positive cases were detected in SDC, LGCCC and CAC, while none of them harbored ETV6 gene rearrangement, indicating that MMG may not serve as a promising diagnostic marker for MASC,and molecular study is needed to distinguish MASC from other salivary gland carcinomas.

关 键 词：乳腺球蛋白 乳腺样分泌癌 唾液腺肿瘤 ETV6基因重排 

分 类 号：R739.87[医药卫生—肿瘤]