唾液腺黏液表皮样癌中CLIC3的表达及意义  

Expression and significance of CLIC3 in salivary gland mucoepidermoid carcinoma

作　　者：王智明[1] 温惠媛 叶明[2] 杨东升[1] 张维庆[1] 潘遥 马颖[3] 

机构地区：[1]中国医科大学附属盛京医院口腔颌面外科,辽宁沈阳110004 [2]抚顺矿务局总医院口腔科,辽宁抚顺113008 [3]中国医科大学附属盛京医院病理科,辽宁沈阳110004

出　　处：《中国口腔颌面外科杂志》2016年第3期239-243,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：辽宁省科学技术计划项目(2012225100)

摘　　要：目的 :探讨细胞内氯离子通道蛋白3(chloride intracellular channel protein 3,CLIC3)在唾液腺黏液表皮样癌(mucoepidermoid carcinoma,MEC)中的表达及临床意义。方法 :采用免疫组织化学和Western免疫印迹方法检测MEC细胞株M3SP2和MC3中CLIC3的表达情况。应用免疫组织化学方法检测49例大唾液腺和18例小唾液腺MEC组织中CLIC3的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:CLIC3在黏液表皮样癌组织中的阳性表达率为83.6%,正常唾液腺组织中无阳性表达(P<0.01)。CLIC3的高表达与唾液腺MEC的临床病理参数之间无显著相关性(P>0.05)。黏液表皮癌细胞株M3SP2和MC3均阳性表达CLIC3蛋白。结论:CLIC3可能在MEC的发生与发展中发挥一定作用。PURPOSE： To investigate the expression and significance of chloride intracellular channel protein 3（CLIC3） in salivary gland mucoepidermoid carcinoma（MEC）. METHODS： Immunohistochemical and Western blot method were used to detect the expression of CLIC3 in MEC M3SP2 and MC3 cell lines. Immunohistochemical method was used to detect the expression of CLIC3 in 49 major salivary glands and 18 minor salivary glands MEC tissues. SPSS 13.0 software package was used for statistical analysis. RESULTS： The positive expression rate of CLIC3 in salivary gland MEC tissues was 83.6%, whereas none in normal salivary gland tissues（P〈0.01）. High expression of CLIC3 was not related to clinicopathological parameters of salivary gland MEC（P〉0.05）. The expression of CLIC3 protein was positive in MEC M3SP2 and MC3 cell lines. CONCLUSIONS： CLIC3 may play a role in carcinogenesis and progress of salivary gland MEC.

关 键 词：细胞内氯离子通道蛋白3 唾液腺 黏液表皮样癌 

分 类 号：R739.87[医药卫生—肿瘤]