18S rRNA作为植物实时荧光定量PCR内参基因的探究  

The Exploration of 18S rRNA for Quantitative RT-PCR as Reference Gene in Plant

作　　者：周晓馥[1] 王晶[1] 史宏伟[1] 徐洪伟[1] 

机构地区：[1]吉林师范大学植物资源科学与绿色生产吉林省重点实验室,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2016年第2期115-119,共5页Journal of Jilin Normal University:Natural Science Edition

基　　金：吉林省科技发展计划项目(20130206059NY)

摘　　要：实时荧光定量PCR(real time fluorescence quantitative PCR,qRT-PCR)已广泛用于基因表达分析,而内参基因的选择对qRT-PCR定量分析的数据校正起关键作用.以18S rRNA作为小麦、苜蓿和杜鹃qRT-PCR的内参基因,探究其表达丰度是否适合作为这3种植物的内参基因.结果表明18S rRNA在这3种植物中的表达丰度均过高,Ct值均小于15,影响目的基因定量的准确性.因此,在目的基因的表达量低时,18S rRNA不宜作为这3种植物的内参基因.Real time fluorescence quantitative PCR（ qRT-PCR） has been widely used for gene expression analysis,and the choice of reference genes plays a key role for the quantitative analysis of qRT-PCR data correction. Here,18 S rRNA was employed as reference gene to explore that if its expression abundance is suitable for wheat,medicago and rhododendron. The results showed that the expression abundance of 18 S rRNA in these three plants were too high with Ct values less than 15,which will have an effect on the quantitative accuracy of the target gene. Therefore,18 S rRNA is not the appropriate reference gene for these three plants when target gene expression is low.

关 键 词：18S RRNA QRT-PCR 内参基因 CT值 表达丰度 

分 类 号：Q943.1[生物学—植物学]