茶树菇液体发酵条件研究  

Study on the Liquid Fermentation Condition of Agrocybeaegerita(Brig.) Sing

作　　者：刘敏[1] 卢红[2] 黄媛媛[2] 荣群[2] 王谦[1] 

机构地区：[1]河北大学生物技术研究中心,河北保定071002 [2]河北大学生命科学学院,河北保定071002

出　　处：《北方园艺》2016年第11期142-144,共3页Northern Horticulture

基　　金：河北省现代农业产业技术体系食用菌创新团队资助项目

摘　　要：以茶树菇为试材,采用液体摇瓶培养法,通过单因素试验、正交实验,以菌丝体生物量为主要指标,对茶树菇液体发酵培养基配方及培养条件进行优化。结果表明:最适宜的培养基配方为葡萄糖3.0%、豆饼粉1.0%、酵母膏0.2%、MgSO_4·7H_2O 0.10%、KH_2PO_40.10%。适宜的茶树菇液体培养条件为起始pH 6.0~7.0、培养温度25~28℃、摇床转速150~180r·min^(-1)。Taking Agrocybe aegeritaas test material,the liquid fermentation condition of Agrocybe aegeritain submerged culture with shake flask were studied according to the index of mycelium fresh weight by single factor experiment and quadrature experiment.The results showed that the optimal liquid culture medium was as follows：glucose 3.0%,soybean cake powder 1.0%,yeast extract 0.2%,MgSO4·7H2O 0.10%,KH2PO4 0.10%.The optimal culture condition were initial pH 6.0-7.0,temperature 25-28℃,shaking rate 150（-1）80r·min（-1）.

关 键 词：茶树菇 液体培养基 培养条件 优化 菌丝体鲜重 

分 类 号：S646.2[农业科学—蔬菜学]