荧光定量PCR检测乙肝DNA分析前质量控制  

Quality control before analysis of fluorescence quantitative PCR detecting HBV-DNA

作　　者：关玲英[1] 

机构地区：[1]新疆维吾尔自治区人民医院临检中心,新疆乌鲁木齐830001

出　　处：《医药前沿》2016年第10期18-19,共2页Journal of Frontiers of Medicine

摘　　要：目的：探讨荧光定量PCR检测乙肝DNA分析前质量控制措施。方法：选取本院收治的25例乙肝两对半检测确诊为乙肝大三阳患者的血液，每个患者的血液平均分为A、B、C、D组，A组即刻测定，B组室温放置2天测定，C组40C放置7天测定，D组溶血即刻测定，比较不同的储存时间、温度以及标本是否溶血对乙肝病毒DNA进行荧光定量测量结果的影响。结果：未溶血标本与溶血标本相比以及不同储存时间和温度相比，差异均无统计学意义（P〉0．05）。结论：标本溶血、储存时间以及温度对荧光定量PCR检测乙肝大三阳血清标本DNA的结果无影响。Objective To investigate the measure of quality control before analysis of fluorescence quantitative PCR detecting HBV-DNA. Methods Select our hospital 25 cases of patients diagnosed with hepatitis B positive blood, the blood of each patient were divided into A, B, C, D groups. A group immediately determined, group B at room temperature for 2 days before determination, group C at 4℃ for 7days before measured, D hemolytic group measured immediately. Compare whether different storage time, temperature and the effect of sample hemolysis affect the measurements of fluorescence quantitative HBV-DNA. Results No hemolysis compared with hemolysis, different storage time and temperature compared with each other, the difference was not statistically significant （P 〉 0.05）. Conclusion Hemolysis, storage time and temperature have no effect on the fluorescence quantitative PCR analysis of DNA samples of serum hepatitis B patients.

关 键 词：荧光定量PCR 乙肝DNA 分析前质量控制 

分 类 号：R575[医药卫生—消化系统]