锰离子增强MRI活体视神经示踪技术研究进展  

Research progress of Manganese-enhanced MRI optic nerve tracing in vivo

作　　者：陈瑶 胡运韬 马志中 

机构地区：[1]北京清华长庚医院眼科,北京102218

出　　处：《中华实验眼科杂志》2016年第6期562-565,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：国家重点基础研究发展规划（973计划）项目（2011CB707506）

摘　　要：锰离子增强MRI（MEMRI）视神经示踪利用Mn2＋可在神经细胞轴索内运输并跨突触传递和其顺磁性特点可反映视神经轴浆流和视路连接情况,被广泛应用于视神经示踪研究,是一种极具潜力的神经示踪技术.Mn2＋通过钙离子门控通道进入细胞后通过不依赖电活动的微管系统的慢性轴浆运输,但其跨突触传递则需要细胞电活动的参与.眼科可利用MEMRI的特点进行视神经损伤相关的研究.Mn2＋属于细胞内MRI增强剂且具有金属毒性,给药宜多次少量靶点给药.就Mn2＋神经示踪的机制、MEMRI在眼科研究中的应用、Mn2＋给药途径及其毒性做一概述.Manganese-enhanced MRI （MEMRI） optic nerve tracing in vivo is a technology which can reflect the axonal transport status and visual pathway connection by utilizing divalent manganese ion＆#39;s transportability in axons and its characteristics of paramagnetism,and such potential optic nerve tract-tracing technique has been widely used in ophthalmologic research.Mn2＋ enters the neurons through the voltage-gating Ca2＋ channel and is transported by microtubule system which is not depended on cellular electric activity.However,the trans-synaptic transport of Mn2＋ relies on membrane potential.Taking advantages of MEMRI,optic nerve injury-related research could be implemented.Mn2＋ is an intracellular contrast agent with metal toxicity,so low dose,fractionated,targeted delivery of Mn2＋ is recommended to alleviate the adverse effects of Mn2＋.Herein,the mechanism of divalent manganese ion optic nerve tracing,application of MEMRI in ophthalmologic research field,drug delivery approach of divalent Mn2＋ and its toxicity were summarized.

关 键 词：锰离子 磁共振 视神经 

分 类 号：R77[医药卫生—眼科]