不同干燥方法对滇重楼总皂苷含量和抗氧化活性的影响  

Effects of Different Drying Methods on Total Saponin Content and Antioxidant Capacity of Paris polyphylla var. yunnanensis

作　　者：张静[1] 丁博[1] 张华[1] 祁俊生[1] 沈昱翔[2] 周浓[1] 陈秀红[1] 杨冬梅[1] 

机构地区：[1]重庆三峡学院生命科学与工程学院,重庆404000 [2]安顺学院农学院,贵州安顺561000

出　　处：《中国中医药信息杂志》2016年第7期95-97,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81260622);贵州省科技厅科学技术基金[黔科合J字LKA(0122)07];万州区科技计划项目(201301024);重庆市教委科学技术研究项目(KJ131109)

摘　　要：目的研究9种不同干燥方法对滇重楼根茎中总皂苷含量的影响及抗氧化性的差异。方法采用紫外-可见分光光度法测定滇重楼药材中总皂苷的含量,通过1,1-二苯基-2-苦基肼(DPPH)法、2,2'-连氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS^+)自由基清除法评价其皂苷提取物的抗氧化能力。结果不同干燥方法滇重楼根茎中总皂苷含量存在显著差异,35℃烘干样品的总皂苷含量最高,为17.557 mg/g,其次为自然阴干样品13.740 mg/g。综合2种抗氧化能力检测结果,不同干燥方法滇重楼皂苷提取物均有很好的抗氧化能力,其中35℃烘干样品抗氧化能力最强,均表现一定的浓度依赖效应。结论 35℃烘干干燥处理是获得高品质和高活性滇重楼药材的较好方法。ObjectiveTo study the effects of 9 different drying methods on total saponin content and antioxidant capacity ofParis polyphylla var.yunnanensis.Methods UV-VIS spectrophotometry was used for measuring the total saponin content ofP. polyphylla var.yunnanensis, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiozoline-6)-sulphonic acid (ABTS+) radical scavenging methods were used for evaluating the antioxidant capacity ofP. polyphylla var. yunnanensisextracts.ResultsThe total saponin contents ofP. polyphylla var.yunnanensis dried by different methods existed significant difference. The sample dried at 35℃ showed highest total saponin content (17.557 mg/g). The sample dried in the shade naturally showed middle total saponin content (13.740 mg/g). Based on the results of antioxidant capacity detected by two methods, the samples ofP. polyphylla var. yunnanensis dried by 9 different methods all showed good antioxidant capacity and showed a certain concentration dependence.Conclusion The samples dried at 35℃ have the highest antioxidant capacity. Therefore, drying at 35℃ is a better drying method to obtain high quality and high activity ofP. polyphylla var.yunnanensis.

关 键 词：滇重楼 干燥方法 烘干法 总皂苷 抗氧化活性 

分 类 号：R283.1[医药卫生—中药学]